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Table 1 Comparison of miRNA expression using MiRCURY array and TaqMan miRNA assay

From: Profiling microRNA expression in Arabidopsis pollen using microRNA array and real-time PCR

 

MiRCURY Array£

Taqman MiRNA Assay¥

   
 

log2(sample/pool)

MP CT

Inf CT

 

AS

Slotkin et. al€

Name

Inf 1

Inf 2

MP1

MP2

mean

mean

ΔΔCT

RP§

Inf

MP

156a-f

-0.44

0.27

0.23

0.19

29.85

29.77

-0.9

2

2591

51961

156g

-0.33

0.34

0.16

0.09

35.08

34

0.09*

0

131

678

156h

    

35.49

31.6

2.89

0

1525

611

157a-d

-0.32

0.92

-1.35

-1.04

34.42

31.18

2.26

1

1792

671

158a

0.07

0.77

-2.21

-2.12

32.87

27.9

3.95

8

16305

2307

158b

0.15

0.79

-1.82

-1.82

32.89

30.5

1.39

0

13757

2037

159a

-0.33

1.03

-3.17

-3.04

NA

25.2

 

67

197957

5540

159b

-0.31

1.04

-3.31

-3.01

NA

24.3

 

10

189644

5319

159c

-0.17

1.07

-2.87

-2.84

35.85

29.7

4.49

0

35850

1247

160a-c

-0.04

0.39

-0.31

-0.39

30.71

28.95

0.74

17

833

351

161

-0.03

1.04

-1.29

-1.36

33.65

29.7

2.91

71

8245

4737

162a,b

0.05

0.73

-1.13

-1.2

35.35

28.9

5.82

7

123

35

163

0.39

0.51

-3.27

-2.83

36.98

30.5

5.29

3

5665

126

164a,b

NA

NA

NA

NA

36.39

25.6

8.53

21

10771

382

164c

NA

NA

NA

NA

37.04

26.1

10.03

1

10005

349

165a,b

0.31

0.58

0

0.06

   

1

1

15

166a-g

-0.24

1.11

-3.04

-3.25

31.93

24.9

6.4

6

472

33

167a,b

-0.64

0.96

-2.4

-2.29

35.95

25.6

9.48

218

33681

1154

167c

-0.34

0.94

-1.41

-1.55

NA

26.1

 

1

250

14

167d

-0.09

0.85

-0.76

-0.96

NA

34

 

4

852

19

168a,b

0.37

0.36

-0.7

-1.18

32.49

28.6

3.56

16

19462

5038

169a

0.07

0.83

-0.91

-0.94

NA

33.8

 

1

399

7

169b,c

NA

NA

NA

NA

NA

32.5

 

0

380

7

169d-g

-0.01

0.52

-0.74

-0.98

NA

30.01

 

2

234

9

170

NA

NA

NA

NA

32.9

29.3

2.95

7

5207

137

171a

0

0.79

-2.7

-2.67

37.0

26.5

9.81

247

39570

922

171b,c

0.14

0.51

-1.6

-1.52

36.28

26.8

8.40

5

4149

91

172a,b

0.08

0.79

-1.47

-1.55

32.69

28.9

3.14

73

8220

8147

172c,d

NA

0.26

-0.36

-0.33

32.47

28.4

3.47

1

7576

8353

172e

0.04

0.75

-1.42

-1.48

32.81

28.9

3.17

6

5245

2320

173

-0.38

0.39

-1.99

-1.93

34.93

29.6

4.71

1

625

501

319a,b

0.07

0.33

-0.93

-0.97

36.18

27.44

8.26

5

14514

713

319c

    

36.97

30.11

6.38

2

13795

653

390a,b

0.14

0.66

-1.39

-1.33

33.15

24.86

7.79

2

8743

695

391

0.15

0.42

-0.6

-0.54

   

3

14

19

394a,b

NA

0.42

-0.93

-0.96

35.84

28.97

6.43

1

21

1

395a,d,e

-0.04

0.11

-0.49

-0.47

NA

34.91

 

0

139

2

395b,c,f

-0.09

0.22

0.01

-0.07

NA

31.94

 

0

137

2

396a

NA

0.72

NA

-0.76

34.87

29.34

4.91

6

352

19

396b

0.06

1.18

-0.92

-1.39

NA

27.5

 

4

371

21

399b,c

-0.21

0.09

-0.31

NA

35.84

32.12

2.91

0

485

106

403

NA

NA

NA

NA

36.61

30.9

4.92

1

696

275

414

0.18

0.11

-0.42

-0.66

   

NA

0

0

419

0.17

0.31

-0.47

-0.32

NA

NA

 

NA

0

0

447a,b

-0.13

0.11

-0.04

-0.15

   

0

286

49

447c

-0.26

-0.1

0.22

0.23

NA

NA

 

0

0

0

  1. All miRNA names have been abbreviated. If a probe represents a miRNA family with more than three members, the name is shortened to save space. For example, miR156a-f represents miR156a, b, c, d, e, f. MiRNAs that were not present either on the array or the Taqman assay were in bold. £, the MiRCURY array data were shown as Log2(sample/pool). ¥, The CT value represents means from all the replicates. T-test was performed to evaluate the ΔΔCT value for difference that is statically significant (with p < 0.05). The only one that did not pass the test was marked by an asterisk. §, the value in the table represents the normalized read (reads/million) for each miRNA in inflorescence (Col-0) as stored in ASRP database. €, the value represents the un-normalized sequencing reads from the small RNA libraries published by Slotkin et. al. [29]. The whole inflorescence library has 4,158,848 sequences, while the mature pollen library has 1,034,665 sequences. The table only listed miRNAs that were found in at least one of the sources. Inf, inflorescence; MP, mature pollen. NA, the miRNA was either not detected or unavailable.