Figure 1

Raffinose synthase amino acid sequence alignments in the regions surrounding the induced mutations in the RS2 gene. Amino acid positions are indicated at the beginning of each alignment. The position of the polymorphic amino acid is indicated by an asterisk. Identical amino acid residues are highlighted in black while similar amino acid residues are highlighted in gray. A. The exon one region containing the induced mutation in line 165 which resulted in S150F. B. The exon one region containing the induced mutation in line 397 which resulted in T107I. C. Weblogo output of the amino acid conservation of raffinose synthase enzymes aligned as part of the BLINK feature at NCBI http://0-www-ncbi-nlm-nih-gov.brum.beds.ac.uk/staff/tao/URLAPI/BLINK_tut2.html using GI number 187610414. Amino acid positions within the protein are listed on the X axis. The overall height for each amino acid column stack indicates the sequence conservation at that position while the height of one-letter amino acid symbols within the column stack indicates the relative frequency of each amino acid in that position [23].