- Research article
- Open Access
Differential effectiveness of Serratia plymuthica IC1270-induced systemic resistance against hemibiotrophic and necrotrophic leaf pathogens in rice
© De Vleesschauwer et al; licensee BioMed Central Ltd. 2009
Received: 03 September 2008
Accepted: 22 January 2009
Published: 22 January 2009
Induced resistance is a state of enhanced defensive capacity developed by a plant reacting to specific biotic or chemical stimuli. Over the years, several forms of induced resistance have been characterized, including systemic acquired resistance, which is induced upon localized infection by an avirulent necrotizing pathogen, and induced systemic resistance (ISR), which is elicited by selected strains of nonpathogenic rhizobacteria. However, contrary to the relative wealth of information on inducible defense responses in dicotyledoneous plants, our understanding of the molecular mechanisms underlying induced resistance phenomena in cereal crops is still in its infancy. Using a combined cytomolecular and pharmacological approach, we analyzed the host defense mechanisms associated with the establishment of ISR in rice by the rhizobacterium Serratia plymuthica IC1270.
In a standardized soil-based assay, root treatment with IC1270 rendered foliar tissues more resistant to the hemibiotrophic pathogen Magnaporthe oryzae, causal agent of the devastating rice blast disease. Analysis of the cytological and biochemical alterations associated with restriction of fungal growth in IC1270-induced plants revealed that IC1270 primes rice for enhanced attacker-induced accumulation of reactive oxygen species (ROS) and autofluorescent phenolic compounds in and near epidermal cells displaying dense cytoplasmic granulation. Similar, yet more abundant, phenotypes of hypersensitively dying cells in the vicinity of fungal hyphae were evident in a gene-for-gene interaction with an avirulent M. oryzae strain, suggesting that IC1270-inducible ISR and R protein conditioned effector-triggered immunity (ETI) target similar defense mechanisms. Yet, this IC1270-inducible ISR response seems to act as a double-edged sword within the rice defense network as induced plants displayed an increased vulnerability to the necrotrophic pathogens Rhizoctonia solani and Cochliobolus miyabeanus. Artificial enhancement of ROS levels in inoculated leaves faithfully mimicked the opposite effects of IC1270 bacteria on aforementioned pathogens, suggesting a central role for oxidative events in the IC1270-induced resistance mechanism.
Besides identifying ROS as modulators of antagonistic defense mechanisms in rice, this work reveals the mechanistic similarities between S. plymuthica-mediated ISR and R protein-dictated ETI and underscores the importance of using appropriate innate defense mechanisms when breeding for broad-spectrum rice disease resistance.
Plants have evolved a powerful immune system to resist their potential colonization by microbial pathogens and parasites. Over the past decade, it has become increasingly clear that this innate immunity is, in essence, composed of two interconnected branches, termed PAMP-triggered immunity (PTI) and effector-triggered immunity (ETI) [1, 2]. PTI is triggered by recognition of pathogen- or microbial-associated molecular patterns (PAMPs/MAMPs), which are conserved molecular signatures decorating many classes of microbes, including non-pathogens. Perception of MAMPs by pattern recognition receptors (PRRs) at the cell surface activates a battery of host defense responses leading to a basal level of resistance . As a result of the evolutionary arms-race between plants and their intruders, many microbial pathogens acquired the ability to dodge PTI-based host surveillance via secretion of effector molecules that intercept MAMP-triggered defense signals . In turn, plants have adapted to produce cognate R-(resistance) proteins by which they recognize, either directly or indirectly, these pathogen-specific effector proteins, resulting in a superimposed layer of defense variably termed effector-triggered immunity (ETI), gene-for-gene resistance or R-gene-dependent resistance .
In many cases, effector recognition culminates in the programmed suicide of a limited number of challenged host cells, clearly delimited from the surrounding healthy tissue. This hypersensitive response (HR) is thought to benefit the plant by restricting pathogen access to water and nutrients and is correlated with an integrated set of physiological and metabolic alterations that are instrumental in impeding further pathogen ingress, among which a burst of oxidative metabolism leading to the massive generation of reactive oxygen species (ROS) [5, 6]. Apart from local immune responses, ETI-associated HR formation also mounts a long-distance immune response termed systemic acquired resistance (SAR), in which naïve tissues become resistant to a broad spectrum of otherwise virulent pathogens . It should be noted, however, that PTI, when activated by PAMPs that activate the SA signaling pathway, can trigger SAR as well .
An archetypal inducible plant defense response, SAR requires endogenous accumulation of the signal molecule salicylic acid (SA) and is marked by the transcriptional reprogramming of a battery of SA-inducible genes encoding pathogenesis-related (PR) proteins. By contrast, there is ample evidence for induced disease resistance conditioned by molecules other than SA, as illustrated by rhizobacteria-mediated induced systemic resistance [ISR; ]. ISR, which delivers systemic protection without the customary pathogenesis-related protein induction, is a resistance activated upon root colonization by specific strains of plant growth-promoting rhizobacteria (PGPRs) . In a series of seminal studies using the reference strain Pseudomonas fluorescens WCS417r, Pieterse and associates [11–13] demonstrated that, at least in Arabidopsis, ISR functions independently of SA, but requires components of the jasmonic acid (JA) and ethylene (ET) response pathways. Even though colonization of the roots by ISR-triggering bacteria leads to a heightened level of resistance against a diverse set of intruders, often no defense mechanisms are activated in aboveground plant tissues upon perception of the resistance-inducing signal. Rather, these tissues are sensitized to express basal defense responses faster and/or more strongly in response to pathogen attack, a phenomenon known as priming . As demonstrated recently, priming of the plant's innate immune system confers broad-spectrum resistance with minimal impact on seed set and plant growth . Hence, priming offers a cost-efficient resistance strategy, enabling the plant to react more effectively to any invader encountered by boosting infection-induced cellular defense responses [16, 17].
In contrast to the overwhelming amount of information on inducible defenses in dicotyledonous plant species, our understanding of the molecular mechanisms underpinning induced disease resistance in rice (Oryza sativa) and other cereals is still in its infancy . Evidence demonstrating that central components of the induced resistance circuitry, including the master regulatory protein NPR1, are conserved in rice has only recently been presented [19–22]. Moreover, reports on SAR-like phenomena in rice are scarce. Most tellingly in this regard, a 17-year-old report of systemically enhanced resistance against the rice blast pathogen M. oryzae triggered by a localized infection with the non-rice pathogen P. syringae pv. syringae remains one of the most compelling examples of a SAR-like response in rice to date . In contrast, there is a sizeable body of evidence demonstrating systemic protection against various rice pathogens resulting from ISR elicited by, amongst others, Pseudomonas [24, 25], Bacillus  and Serratia strains . However, in most if not all cases, still very little is known about the basic mechanisms governing this ISR response.
In a previous report, we demonstrated that rice plants of which the roots were colonized by the fluorescent pseudomonad P. aeruginosa 7NSK2 developed an enhanced defensive capacity against infection with M. oryzae. Bacterial mutant analysis revealed that this 7NSK2-mediated ISR is based on secretion of the redox-active pigment pyocyanin. Perception of pyocyanin by the plant roots was shown to cue the formation of reiterative micro-oxidative bursts in naïve leaves, thereby priming these leaves for accelerated expression of HR-like cell death upon pathogen attack . Aiming to gain further insight into the molecular mechanisms underpinning rhizobacteria-modulated ISR in rice, we tested the ability of the biocontrol agent Serratia plymuthica IC1270 to induce systemic resistance against various rice pathogens with different modes of infection. Originally isolated from the rhizosphere of grapes,S. plymuthica IC1270 is a well-characterized PGPR strain producing a broad palette of antimicrobial compounds [29–32]. In addition to its potential as a direct antagonist of a wide array of plant pathogens, preliminary experiments in bean and tomato revealed that IC1270 is equally capable of reducing disease through activation of a plant-mediated defense response . Here, we demonstrate that colonization of rice roots by IC1270 renders foliar tissues more resistant to M. oryzae. Using a combined cytological and pharmacological approach, evidence is provided that IC1270 locks plants into a pathogen-inducible program of boosted ROS formation, culminating in the prompt execution of HR cell death at sites of attempted pathogen entry. Similar, yet even more pronounced, phenotypes of hypersensitively dying cells in the vicinity of fungal hyphae were observed in a genetically incompatible rice-M. oryzae interaction, suggesting that IC1270-mediated ISR and R-gene-mediated ETI involve similar defense mechanisms. However, this IC1270-inducible ISR seems to play an ambivalent role within the rice disease resistance network, as bacteria-treated plants were rendered hypersusceptible to the necrotrophic pathogens R. solani and C. miyabeanus
Cultivation of rhizobacteria and pathogens
Bacterial strains used in this study were Serratia plymuthica IC1270, which was originally described as Enterobacter agglomerans , and Pseudomonas aeruginosa 7NSK2 . For inoculation experiments, IC1270 and 7NSK2 were grown on iron-limiting King's B medium [KB; ] for 24 h at 28°C and 37°C, respectively. Bacterial cells were scraped off the plates and suspended in sterile saline (0.85% NaCl). Densities of the bacterial suspensions were adjusted to the desired concentration based on their optical density at 620 nm.
Magnaporthe oryzae isolate VT7, a field isolate from rice in Vietnam , was grown at 28°C on half-strength oatmeal agar (Difco, Sparks, USA). Seven-day-old mycelium was flattened onto the medium using a sterile spoon and exposed to blue light (combination of Philips TLD 18W/08 and Philips TLD 18W/33) for seven days to induce sporulation. Conidia were harvested as described in De Vleesschauwer et al. , and inoculum concentration was adjusted to a final density of 1 × 104 spores ml-1 in 0.5% gelatin (type B from Bovine skin; Sigma-Aldrich G-6650).
Rhizoctonia solani isolate MAN-86, belonging to anastomosis group AG-1 IA , was maintained on potato dextrose agar (PDA; Difco Laboratories, Detroit, USA). Inoculum was obtained according to Rodrigues et al.  with minor modifications. After autoclaving, 15 toothpicks, 1 cm in length, and five agar plugs (5 mm in diameter), obtained from the margin of an actively growing colony of R. solani, were transferred to PDA plates. These plates were then incubated for 8 days at 28°C so R. solani could colonize the toothpicks.
Cochliobolus miyabeanus strain 988, obtained from diseased rice in field plots at the International Rice Research Institute (Manila, The Philippines), was grown for sporulation at 28°C on PDA. Seven-day-old mycelium was flattened onto the medium using a sterile spoon and exposed to blue light for three days under the same conditions mentioned above. Upon sporulation, conidia were harvested exactly as stated in Thuan et al.  and re-suspended in 0.5% gelatin to a final density of 1 × 104 conidia ml-1.
Pathogen inoculation and disease rating
Four-week-old rice seedlings (5-leaf stage) were challenge-inoculated with Magnaporthe oryzae as described in De Vleesschauwer et al. . Six days after inoculation, disease severity on the fourth leaves of each plant was rated by counting the number of elliptical to round-shaped lesions with a sporulating gray center, and expressed relative to non-induced control plants.
R. solani bioassays were performed essentially as described in Rodrigues et al. . Plants were challenged when four weeks old by placing a 1-cm toothpick colonized by R. solani inside the sheath of the second youngest fully expanded leaf. Inoculated plants were maintained inside humid inoculation chambers (≥ 92% relative humidity; 30 ± 4°C) for 72 h, and, thereafter, transferred to greenhouse conditions. Four days after challenge infection, disease severity was assessed by measuring the length of the water-soaked lesions.
C. miyabeanus bioassays were performed as described in Ahn et al.  with minor modifications. Five-week-old seedlings (6.5-leaf stage) were misted with a C. miyabeanus spore suspension containing 1 × 104 conidia ml-1 in 0.5% gelatin. Inoculated plants were kept in a dew chamber (≥ 92% relative humidity; 30 ± 4°C) for 18 h to facilitate fungal penetration, and subsequently transferred to greenhouse conditions for disease development. Disease symptoms were scored at four days after inoculation for about 48 leaves per treatment. Disease ratings were expressed on the basis of diseased leaf area and lesion type: I, no infection or less than 2% of leaf area infected with small brown specs less than 1 mm in diameter; II, less than 10% of leaf area infected with brown spot lesions with gray to white center, about 1–3 mm in diameter; III, average of about 25% of leaf area infected with brown spot lesions with gray to white center, about 1–3 mm in diameter; IV, average of about 50% of leaf area infected with typical spindle-shaped lesions, 3 mm or longer with necrotic gray center and water-soaked or reddish brown margins, little or no coalescence of lesions; V, more than 75% of leaf area infected with coalescing spindle-shaped lesions.
Induced systemic resistance (ISR) assays were performed as described in De Vleesschauwer et al.  with minor modifications. Briefly, rice plants (Oryza sativa spp. indica line CO39) were grown under greenhouse conditions (30 ± 4°C, 16-h photoperiod) in commercial potting soil (Structural; Snebbout, Kaprijke, Belgium) that had been autoclaved twice on alternate days for 21 min. Rice seeds first were surface sterilized with 1% sodium hypochlorite for two min, rinsed three times with sterile, demineralized water and incubated for five days on a wet sterile filter paper in sealed Petri dishes at 28°C. Prior to sowing in perforated plastic trays (23 by 16 by 6 cm), roots of germinated seeds were dipped in a bacterial suspension of the ISR-inducing strains [5 × 107 colony-forming units (cfu) ml-1] for 10 min. The autoclaved soil was thoroughly mixed with bacterial inoculum to a final density of 5 × 107 cfu ml-1. To ensure consistent root colonization by the eliciting bacteria, rice plants were soil-drenched a second time with bacterial inoculum (5 × 107 cfu ml-1) at ten days after sowing. In control treatments, soil and rice plants were treated with equal volumes of sterilized saline.
For experiments in which purified pyocyanin was applied to the roots of rice seedlings, plants were grown in a hydroponic gnotobiotic system as described before . In this system, plants were fed with various concentrations of pyocyanin and ascorbate 4 days before challenge inoculation by adding the desired concentration to the half-strength Hoagland nutrient solution. Pyocyanin extraction, quantification and application were performed exactly as stated in De Vleesschauwer et al. .
Evaluation of plant colonization by S. plymuthica IC1270 and P. aeruginosa7NSK2
Bacterial colonization of the plant roots was determined by the time the bioassays were discontinued. Roots of three plants of each treatment were rinsed to remove most of the soil, weighed, and 1 g of root was macerated in sterile demineralized water. Serial dilutions were plated on KB agar supplemented with rifampicin (40 μg/ml) for IC1270, and KB agar for 7NSK2. After overnight incubation at 28°C and 37°C for IC1270- and 7NSK2-treated roots, respectively, the number of colony-forming units per gram of root fresh weight was determined. Possible spreading of root-inoculated bacteria to distal leaves was checked as described before . The detection limit of this assay is approximately 10 CFU per sheath or leaf blade.
Cytological analysis of IC1270-mediated ISR against M. oryzae
To gain more insight into the nature of IC1270-mediated ISR against M. oryzae, cytological studies were performed at sites of pathogen entry. To this purpose, we adopted the intact leaf sheath assay previously described by Koga et al. . Briefly, leaf sheaths of the fifth leaf of rice plants at the 5.5 leaf stage were peeled off with leaf blades and roots. The leaf sheath was laid horizontally on a support in plastic trays containing wet filter paper, and the hollow space enclosed by the sides of the leaf sheaths above the mid vein was filled with a suspension of spores (5 × 104 conidia ml-1) of M. oryzae. Inoculated leaf sheaths were then incubated at 25°C with a 16-h photoperiod. When ready for microscopy, the sheaths were hand-trimmed to remove the sides and expose the epidermal layer above the mid vein. Lower mid vein cells were removed to produce sections three to four cell layers thick. At least five trimmed sheath tissue sections originating from different control and IC1270-treated plants were used for each sampling point.
Phenolic compounds were visualized as autofluorescence under blue light epifluorescence (Olympus U-MWB2 GPF filter set-excitation: 450 to 480 nm, dichroic beamsplitter; 500 nm, barrier filter BA515). To detect H2O2 accumulation, staining was performed according to the protocol of Thordal-Christensen et al.  with minor modifications. Six hours before each time point, trimmed sheath segments were vacuum-infiltrated with an aqueous solution of 1 mg ml-1 3,3'-diaminobenzidine(DAB)-HCL (pH = 3.8) for 30 min. Thereafter, infiltrated segments were incubated in fresh DAB solution until sampling. DAB polymerizes in the presence of H2O2 and endogenous peroxidase to form a brownish-red precipitate that can be easily visualized using bright-field microscopy. After staining, trimmed sheath segments were mounted in 50% glycerol. Images were acquired digitally (Olympus Color View II camera, Aartselaar, Belgium) and further processed with the Olympus analySIS cell^F software.
Artificial manipulation of the oxidative burst in detached rice leaves
For experiments in which plants were treated with the ROS-generating mixtures glucose plus glucose oxidase (G/GO) and xanthine plus xanthine oxidase (X/XO), fifth-stage leaves of four-week-old rice plants were excised and cut into 7-cm segments. Aspergillus niger glucose oxidase (Sigma-Aldrich, St. Louis, MO) was added to 2 mM D-glucose in 20 mM Na phosphate buffer, pH 6.5, immediately prior to plant treatment (100 units ml-1). Similarly, xanthine oxidase (0.1 units ml-1) was added to 1 mM xanthine in the same buffer solution (Sigma-Aldrich, St. Louis, MO). The ROS-generating mixtures, buffer alone or buffer containing glucose (2 mM), gluconate (50 μM), glucose oxidase (100 units ml-1), xanthine (1 mM), or xanthine oxidase (0.1 units ml-1) were infiltrated in approximately 20 μl aliquots into five sites on the abaxial surface of the detached leaf segments using a syringe without a needle. Alternatively, detached leaf segments were infiltrated with 3-aminotriazole (10 mM) or catalase (1100 units ml-1) in 10 mM MES buffer, pH 6.5. In planta H2O2 generation by G/GO, X/XO, or 3-aminotriazole was visually confirmed by means of abovementioned DAB staining procedure. Upon infiltration, detached leaf segments were immediately placed onto a glass slide in 14.5 × 14.5 cm Petri dishes lined with moist filter paper. Two hours later, 10 μl of M. oryzae or C. miyabeanus conidial suspension (5 × 104 sp ml-1 in 0.25% gelatin) was drop-inoculated in the center of the infiltrated regions. Control leaves were mock-inoculated with a 0.25% (wt vol-1) gelatin suspension. After 24 h, the droplets were removed with a laboratory tissue. For challenge with R. solani, a 0.8-cm-diameter mycelial disc of a 7-day-old PDA culture of R. solani strain MAN-86 was carefully placed in the center of the infiltrated region. As a control, leaf segments were inoculated with a PDA plug without hyphae. Petri dishes with inoculated leaf segments were routinely placed on a laboratory bench and maintained at 21°C to 26°C with a 16 h photoperiod. For M. oryzae and C. miyabeanus assays, disease development was assessed 96 h post-inoculation using digital image analysis (APS assess software; Lakhdar Lamari, Winnipeg, Canada) for quantification of necrotic leaf areas. These areas were represented as the number of pixels and expressed as a percentage of the total pixel number in a fixed 1 cm2-leaf quadrant. In case of R. solani inoculation, disease ratings were visually graded into five classes based on the leaf area affected; 1 = no infection, 2 = 1 to 10%, 3 = 11 to 25 %, 4 = 26 to 50%, and 5 = more than 50% of leaf area affected.
Differential effectiveness of ISR triggered by S. plymuthicaIC1270
To assess the ISR-triggering capacity of S. plymuthica IC1270, susceptible rice plants were grown in soil containing IC1270 bacteria, and subsequently challenged with several fungal pathogens exhibiting different modes of infection. In these ISR bioassays, the resistance-inducing potential of IC1270 was compared to that of P. aeruginosa 7NSK2, a well-studied PGPR strain which we previously uncovered as a potent activator of induced resistance responses in rice .
Because IC1270 clearly inhibited the growth of M. oryzae in dual culture experiments (data not shown), possible systemic plant colonization by the rhizobacteria was checked. However, in all bioassays performed, IC1270 bacteria were absent from sheaths or leaves of root-induced plants, indicating that bacterial colonization remained confined to the root zone (data not shown). Although such spatial separation does not rule out the possibility that IC1270-conferred protection might result from long-distance translocation of bacteria-produced allelochemicals to systemic leaves, the latter is rather unlikely as pilot experiments aimed at elucidating the bacterial traits underpinning IC1270-ISR revealed that mutants defective in the global response regulator protein GacA , which controls the synthesis of various antifungal metabolites (e.g. chitinases and pyrrolnitrin), were as effective as wild-type IC1270 in reducing rice blast disease severity (De Vleesschauwer and Höfte, unpublished results). The cumulative data therefore strongly suggest that the beneficial protective activity exerted by S. plymuthica IC1270 is based on activation of the plant's defensive repertoire, rather then being caused by microbial antagonism.
To test the spectrum of effectiveness of this IC1270-mediated ISR, we next assayed for induction of resistance against the sheath blight pathogen, Rhizoctonia solani, and the brown spot pathogen, Cochliobolus miyabeanus, both of which are considered necrotrophic fungi. In contrast to M. oryzae, which sequentially invades living cells , R. solani and B. oryzae kill host cells at very early stages in the infection, leading to extensive tissue damage . As shown in Fig 1B, both IC1270 and 7NSK2 failed to reduce disease caused by R. solani. This impaired ISR response was not due to insufficient root colonization as bacterial counts in the rhizosphere of treated rice seedlings were comparable to those obtained in the M. oryzae bioassays (1.14 ± 0.19 × 105 CFU. g-1). Interestingly, in all four independent experiments, IC1270 pretreatment favored subsequent infection by R. solani, causing an average 39.6% increase in disease severity relative to non-induced controls. A similar trend was observed when challenging with C. miyabeanus, with IC1270 consistently promoting vulnerability to the latter pathogen (Fig. 1C). Root colonization by 7NSK2, however, yielded variable results. No significant differences between control and 7NSK2-treated plants could be observed in three bioassays, whereas in the two remaining assays, root treatment with 7NSK2 rendered rice seedlings substantially more susceptible to brown spot.
In all experiments, mock-inoculated control plants remained healthy, and no apparent differences in appearance, size, or weight of control, 7NSK2 or IC1270-treated plants were observed prior to challenge infection (data not shown). Thus, under the experimental conditions used in this study, root treatment with the ISR-inducing bacteria did not lead to detectable effects on plant growth that could have affected the growth or development of the respective pathogens.
Collectively, these findings demonstrate that S. plymuthica IC1270 plays an ambivalent role in the rice induced resistance network, acting as a potent elicitor of resistance to the hemibiotroph M. oryzae while promoting susceptibility to the necrotrophs C. miyabeanus and R. solani.
S. plymuthicaIC1270 triggers HR-like responses at the sites of pathogen attack
To begin to unravel the defense mechanism(s) underpinning IC1270-mediated ISR, we analyzed the cytological alterations associated with restriction of M. oryzae in IC1270-induced plants using the intact leaf sheath method designed by Koga and associates . In this system, intact leaf sheaths of control, non-induced and IC1270-treated plants of the highly susceptible rice variety CO39 were routinely inoculated by injecting a conidial suspension of the virulent blast isolate VT7. For comparison with R gene-mediated ETI, we also included the VT7-resistant variety C101LAC, the latter being a near-isogenic line of CO39 carrying the blast resistance genes Pi-1 and Pi-33 [44, 45].
At later stages of infection, M. oryzae had massively colonized the epidermis and mesophyll of CO39 sheaths causing extensive host damage as evidenced by the ubiquitous presence of cellular debris and fragmented remnants of host cell walls around invasive hyphae in the mesophyll (data not shown). By contrast, in resistant C101LAC, as well as in IC1270-induced CO39, invading hyphae were largely trapped within hypersensitively dying cells in the epidermal layer, preventing fungal passage to the underlying tissue.
S. plymuthica IC1270-mediated ISR to M. oryzae involves priming for enhanced attacker-induced H2O2generation
Leaf sheath cells of susceptible CO39 were characterized by the high ratio of H2O2-negative type I reactions, accounting for 78% and 67% of all interaction sites by 36 and 48 hpi, respectively (Fig. 4B). In some incidences (21% of all interaction sites at 48 hpi), H2O2accumulated in the initially penetrated epidermal cell following the formation of an extensively branched mycelium in the neighboring cells. Yet, this type II reaction seemingly occurred too late to effectively stall the pathogen. IC1270-induced CO39 cells, on the other hand, exhibited a strikingly different set of responses in that type I reactions, reaching a level of 33% at 36 hpi, were no longer discernible by 48 hpi. The rapid decline in the frequency of type I reactions from 36 hpi onward corresponded to an approximately 15% increase in the frequency of both type III and type V reactions. HR-like cell death of attacked epidermal cells, seen at approximately 52% of all interaction sites, was always associated with H2O2 accumulation in the cytoplasmic aggregates, beginning 32 hpi. Although not identical, by 48 hpi the H2O2 signature of IC1270-treated CO39 plants showed substantial similarity to that observed in the incompatible interaction between C101LAC and VT7, thereby further emphasizing the possible mechanistic similarities between IC1270-mediated ISR and R-protein-dictated ETI.
Starting 50 hpi, a strong accumulation of H2O2 was found in CO39 mesophyll cells that appeared to collapse, whereas in samples from IC1270-induced CO39 or C101LAC sheaths, DAB staining in the mesophyll layer was seldom observed (data not shown). However, at these late infection stages, massive H2O2 accumulation is most likely a consequence of progressive cellular destruction and overtaxed anti-oxidative capacities, and hence, a chaotic reaction associated with susceptibility, rather than a controlled defense response restricting cellular accessibility for M. oryzae. Together these results clearly demonstrate the potential of IC1270 to prime rice for augmented generation of epidermis-localized H2O2.
Manipulation of oxidative stress in inoculated leaves
Despite the emergence of rice as a pivotal model for molecular genetic studies of disease resistance in cereal crops, molecular information regarding chemically and biologically induced defenses is still largely missing. In an effort to broaden our understanding of the rice induced resistance machinery, we analyzed the host defense responses underpinning ISR triggered by the biocontrol agent S. plymuthica IC1270. The results presented in this study demonstrate that root colonization by IC1270 predisposes rice to undergo a massive oxidative burst and related HR-like cell death at sites of attempted pathogen invasion, a process culminating in heightened resistance to the hemibiotrophic blast pathogen, M. oryzae. The same treatment, however, rendered plants more susceptible to attack by the necrotrophic pathogens R. solani and C. miyabeanus. Besides tagging ROS and HR-like cell death as two-faced players in the rice defense response, these findings strengthen the argument that rice requires distinct mechanisms for defense against M. oryzae and the necrotrophs R. solani and C. miyabeanus.
Mounting evidence indicates that generation of systemic resistance does not necessarily require direct activation of defense mechanisms, but can also result from a faster and stronger activation of basal defenses in response to pathogen attack . For instance, unlike pathogen-induced SAR, classic rhizobacteria-mediated ISR in Arabidopsis is not associated with a direct induction of defense mechanisms, but with priming for augmented defense activation upon challenge inoculation [58, 59]. Other ISR-inducing PGPRs also have been found to enhance the plant's defensive capacity by hyper-activating pathogen-activated defenses [60–64], suggesting that priming for enhanced defense is a common mechanism in PGPR-mediated ISR. The results presented in this study add further support to this concept as root colonization by IC1270 did not cause a strong constitutive resistance phenotype, but rather primed plants to hyper-respond to subsequently inoculated pathogens, resulting in excessive defense activation and enhanced resistance to M. oryzae. This priming effect of IC1270 was borne out by the observation that challenge inoculation of IC1270-colonized plants with M. oryzae entailed a rapid accumulation of autofluorogenic phenolic compounds in and around epidermal cells displaying dense cytoplasmic granulation (Figs. 2 and 3), two features that are considered as hallmarks of an ETI-associated HR [46, 47].
Comparative profiling of pathogenesis-related H2O2 accumulation in blast susceptible, yet ISR-expressing, and genetically resistant leaf sheath cells, further strengthened the parallels between R protein-mediated ETI and IC1270-triggered ISR priming (Fig. 4). Hence, IC1270 appears to protect rice from M. oryzae by reprogramming pathogen-attacked epidermal cells to undergo a rapid HR-like response, thereby providing a possible functional interface between rhizobacteria-mediated ISR and avirulent pathogen-induced ETI. Such mechanistic similarities between ISR and ETI are compatible with the idea that defense signals from multiple 'entry points' can converge and target overlapping sets of defense effectors [65–67]. Particularly relevant in this regard is the substantial overlap between gene expression changes and alterations in SA content induced during an avirulent pathogen-triggered ETI response, and those induced by treatment with flg22, an 22-amino-acid epitope of the archetypal MAMP elicitor flagellin [8, 67, 68]. Although unequivocal evidence is still lacking, the striking homologies with the sensitive perception mechanisms for pathogen-derived MAMPs that function in PTI suggest that ISR-triggering rhizobacteria are recognized in a similar manner [31, 69]. In this perspective, it is not inconceivable that the mechanistic parallels between IC1270-mediated ISR and ETI can be traced to converging MAMP- and R-protein-induced defense responses. Furthermore, consistent with the view of ETI as an accelerated and amplified PTI response [1, 70, 71], such MAMP-orchestrated ISR elicitation may also explain the partial nature of the IC1270-induced resistance against M. oryzae.
Apart from S. plymuthica IC1270, several other biological and chemical agents have been shown to be capable of inducing resistance to M. oryzae [19, 72], among which the SA analog BTH and the redox-active pigment pyocyanin, key determinant of ISR induced by P. aeruginosa 7NSK2 [19, 28]. Interestingly, both these resistance inducers appear to mimic IC1270 in that they produce a similar resistance phenotype, characterized by hypersensitively dying cells in the vicinity of fungal hyphae [28, 38, 73, 74]. Although it does not follow that the signaling conduit(s) governing IC1270-mediated ISR is (are) necessarily the same as that (those) leading to pyocyanin- or BTH-inducible blast resistance, such commonalities apparent at the level of defense mobilization suggest that these elicitors may feed into related, if not identical, resistance pathways. Further supporting this hypothesis is the overlap manifest at the level of resistance to attackers, with IC1270, BTH and pyocyanin all being ineffective or even increasing vulnerability to C. miyabeanus and R. solani [28, 38, 75]. Intriguingly, induction of ISR by the PGPR strain P. fluorescens WCS374r appears to rely on a different resistance strategy and was found to be associated with priming for a diverse set of HR-independent cellular defenses, the prompt elaboration of invading hyphae-embedding tubules being a prominent component . Considering this apparent plasticity in the molecular processes leading to induced resistance against M. oryzae, it is tempting to speculate that rice is endowed with multiple blast-effective induced resistance pathways.
The rapid production of ROS during the so-called oxidative burst is a hallmark of the plant's defense response. Although ROS are generally viewed as initiating agents in the disease resistance network , accumulating evidence indicates that ROS formation can cascade either to the detriment or benefit of the plant depending on the lifestyle and parasitic habits of the invading pathogen [5, 10]. Hence, ROS can play a dual role in pathogen defense, acting as key players in resistance to biotrophic pathogens on the one hand [53, 77], while weakening necrotroph resistance by assisting pathogen-induced host cell death on the other [5, 56, 78, 79]. Taking these facts into account, we propose that priming for enhanced ROS generation may likewise function in IC1270-mediated ISR, thereby accounting for the differential effectiveness of this resistance against hemibiotrophic and necrotrophic pathogen assault. Critical to the formation of a hypothesis of primed ROS generation as a key event in ISR by IC1270 was the observation that artificially increased H2O2 levels, either resulting from infiltration of ROS-generating mixtures, inhibition of endogenous catalase activity or hydroponic feeding of pro-oxidative pyocyanin, faithfully mimicked IC1270 in conditioning resistance to M. oryzae but susceptibility to C. miyabeanus and R. solani. Although we are aware that final proof for primed ROS generation as the causal resistance mechanism underpinning IC1270-mediated ISR requires the use of inhibitor compounds able to abrogate the oxidative burst (e.g. DPI), such scavenger experiments could not be performed since detached leaves, needed for effective infiltration of chemicals in rice, somehow failed to develop ISR. Therefore, we can not rule out the possibility that the altered pathogen response of IC1270-induced plants may result in part from ROS-independent processes. Nonetheless, the involvement of boosted ROS generation in the establishment of IC1270-mediated ISR is apparent.
In accordance with previous studies [56, 80], continuous generation of H2O2 in situ by infiltration of G/GO or 3-AT did not induce any detectable cell death per se, indicating that additional pathogen-induced signals are needed for expression of HR-like cell death. Indeed, current concepts suggest that death of host cells during the HR requires the poised production of nitric oxide (NO) and ROS, coupled to simultaneous suppression of the plant's antioxidant machinery [81–83]. In view of these data, it could be reasoned that IC1270-mediated priming for potentiated ROS generation might lower the threshold for activation of programmed cell death, thereby blocking the hemibiotroph M. oryzae in its initial biotrophic phase. In line with this concept, there is ample evidence demonstrating that early-produced H2O2 is a central signal leading to the elicitation of a wide range of blast-effective defenses, among which programmed cell death. Most tellingly, Kachroo and associates  reported a fungal glucose oxidase gene to sequentially induce H2O2 generation, rapid HR-like cell death and enhanced resistance against M. oryzae when ectopically expressed in young rice plants. On the other hand, it is not inconceivable that IC1270-mediated priming for H2O2 may tilt the ROS-controlled cellular life-or-death balance toward death, thereby facilitating subsequent tissue colonization by the necrotrophs R. solani and C. miyabeanus. This notion is corroborated by recent observations demonstrating that IC1270 pretreatment has no marked impact on the early infection events in C. miyabeanus- or R. solani-challenged plants except for a substantial increase in the number of dying cells preceding the fungal growth front (De Vleesschauwer and Höfte, unpublished results). However, given the myriad defense-related plant responses modulated by ROS [52, 53], other yet unidentified mechanisms also may play a role.
In summary, our results favor a model whereby effective root colonization of rice by IC1270 locks plants into a pathogen-inducible program of boosted ROS generation and prompt execution of HR-like cell death at sites of attempted pathogen invasion, a mechanism which shows remarkable similarity with R protein-mediated ETI responses. Although highly effective against the hemibiotroph M. oryzae, halting the pathogen in its biotrophic phase, IC1270 pretreatment enhanced infection by the necrotrophs R. solani and C. miyabeanus, possibly by facilitating pathogen-triggered host cell death. Considering that defense responses effective against M. oryzae may not be effective against or even assist infection by R. solani and C. miyabeanus, our work underscores the importance of utilizing appropriate innate defense mechanisms when breeding for broad-spectrum rice disease resistance.
This work was supported by a specialization fellowship of the Flemish Institute for the stimulation of Scientific-Technological Research in Industry (IWT, Belgium) given to David De Vleesschauwer and by grants from the Special Research Fund of Ghent University, Belgium.
- Jones JDG, Dangl JL: The plant immune system. Nature. 2006, 444: 323-329. 10.1038/nature05286.PubMedView ArticleGoogle Scholar
- Eulgem T, Somssich IE: Networks of WRKY transcription factors in defense signaling. Curr Opin Plant Biol. 2007, 10: 366-371. 10.1016/j.pbi.2007.04.020.PubMedView ArticleGoogle Scholar
- Chisholm ST, Coaker G, Day B, Staskawicz BJ: Host-microbe interactions: Shaping the evolution of the plant immune response. Cell. 2006, 124: 803-814. 10.1016/j.cell.2006.02.008.PubMedView ArticleGoogle Scholar
- Gohre V, Robatzek S: Breaking the barriers: microbial effector molecules subvert plant immunity. Annu Rev Phytopathol. 2008, 46: 189-215. 10.1146/annurev.phyto.46.120407.110050.PubMedView ArticleGoogle Scholar
- Glazebrook J: Contrasting mechanisms of defense against biotrophic and necrotrophic pathogens. Annu Rev Phytopathol. 2005, 43: 205-227. 10.1146/annurev.phyto.43.040204.135923.PubMedView ArticleGoogle Scholar
- Greenberg JT, Yao N: The role and regulation of programmed cell death in plant-pathogen interactions. Cell Microbiol. 2004, 6: 201-211. 10.1111/j.1462-5822.2004.00361.x.PubMedView ArticleGoogle Scholar
- Durrant WE, Dong X: Systemic acquired resistance. Annu Rev Phytopathol. 2004, 42: 185-209. 10.1146/annurev.phyto.42.040803.140421.PubMedView ArticleGoogle Scholar
- Mishina TE, Zeier J: Pathogen-associated molecular pattern recognition rather than development of tissue necrosis contributes to bacterial induction of systemic acquired resistance in Arabidopsis. Plant J. 2007, 50: 500-513. 10.1111/j.1365-313X.2007.03067.x.PubMedView ArticleGoogle Scholar
- van Loon LC, Bakker P, Pieterse CMJ: Systemic resistance induced by rhizosphere bacteria. Annu Rev Phytopathol. 1998, 36: 453-483. 10.1146/annurev.phyto.36.1.453.PubMedView ArticleGoogle Scholar
- Bostock RM: Signal crosstalk and induced resistance: Straddling the line between cost and benefit. Annu Rev Phytopathol. 2005, 43: 545-580. 10.1146/annurev.phyto.41.052002.095505.PubMedView ArticleGoogle Scholar
- Pieterse CMJ, Van Pelt JA, Ton J, Parchmann S, Mueller MJ, Buchala AJ, Metraux JP, Van Loon LC: Rhizobacteria-mediated induced systemic resistance (ISR) in Arabidopsis requires sensitivity to jasmonate and ethylene but is not accompanied by an increase in their production. Physiol Mol Plant Pathol. 2000, 57: 123-134. 10.1006/pmpp.2000.0291.View ArticleGoogle Scholar
- Pieterse CMJ, van Wees SCM, van Pelt JA, Knoester M, Laan R, Gerrits N, Weisbeek PJ, van Loon LC: A novel signaling pathway controlling induced systemic resistance in Arabidopsis. Plant Cell. 1998, 10: 1571-1580. 10.1105/tpc.10.9.1571.PubMedPubMed CentralView ArticleGoogle Scholar
- Pieterse CMJ, van Wees SCM, Hoffland E, van Pelt JA, van Loon LC: Systemic resistance in Arabidopsis induced by biocontrol bacteria is independent of salicylic acid accumulation and pathogenesis-related gene expression. Plant Cell. 1996, 8: 1225-1237. 10.1105/tpc.8.8.1225.PubMedPubMed CentralView ArticleGoogle Scholar
- Conrath U, Pieterse CMJ, Mauch-Mani B: Priming in plant-pathogen interactions. Trends Plant Sci. 2002, 7: 210-216. 10.1016/S1360-1385(02)02244-6.PubMedView ArticleGoogle Scholar
- van Hulten M, Pelser M, van Loon LC, Pieterse CMJ, Ton J: Costs and benefits of priming for defense in Arabidopsis. Proc Nac Sci USA. 2006, 103: 5602-5607. 10.1073/pnas.0510213103.View ArticleGoogle Scholar
- Beckers GJ, Conrath U: Priming for stress resistance: from the lab to the field. Curr Opin Plant Biol. 2007, 10: 425-431. 10.1016/j.pbi.2007.06.002.PubMedView ArticleGoogle Scholar
- Conrath U, Beckers GJM, Flors V, Garcia-Agustin P, Jakab G, Mauch F, Newman MA, Pieterse CMJ, Poinssot B, Pozo MJ, et al: Priming: Getting ready for battle. Mol Plant Microbe Interact. 2006, 19: 1062-1071. 10.1094/MPMI-19-1062.PubMedView ArticleGoogle Scholar
- Kogel KH, Langen G: Induced disease resistance and gene expression in cereals. Cell Microbiol. 2005, 7: 1555-1564. 10.1111/j.1462-5822.2005.00592.x.PubMedView ArticleGoogle Scholar
- Shimono M, Sugano S, Nakayama A, Jiang CJ, Ono K, Toki S, Takatsuji H: Rice WRKY45 plays a crucial role in benzothiadiazole-inducible blast resistance. Plant Cell. 2007, 19: 2064-2076. 10.1105/tpc.106.046250.PubMedPubMed CentralView ArticleGoogle Scholar
- Yuan YX, Zhong SH, Li Q, Zhu ZR, Lou YG, Wang LY, Wang JJ, Wang MY, Li QL, Yang DL, He Z: Functional analysis of rice NPR1-like genes reveals that OsNPR1/NH1 is the rice orthologue conferring disease resistance with enhanced herbivore susceptibility. Plant Biotechnol J. 2007, 5: 313-324. 10.1111/j.1467-7652.2007.00243.x.PubMedView ArticleGoogle Scholar
- Chern M, Fitzgerald HA, Canlas PE, Navarre DA, Ronald PC: Overexpression of a rice NPR1 homolog leads to constitutive activation of defense response and hypersensitivity to light. Mol Plant Microbe Interact. 2005, 18: 511-520. 10.1094/MPMI-18-0511.PubMedView ArticleGoogle Scholar
- Chern MS, Fitzgerald HA, Yadav RC, Canlas PE, Dong XN, Ronald PC: Evidence for a disease-resistance pathway in rice similar to the NPR1-mediated signaling pathway in Arabidopsis. Plant J. 2001, 27: 101-113. 10.1046/j.1365-313x.2001.01070.x.PubMedView ArticleGoogle Scholar
- Smith JA, Metraux JP: Pseudomonas syringae pv. syringae induces systemic resistance to Pyricularia oryzae in rice. Physiol Mol Plant Pathol. 1991, 39: 451-461. 10.1016/0885-5765(91)90011-6.View ArticleGoogle Scholar
- Nagarajkumara M, Jayaraj J, Muthukrishnan S, Bhaskaran R, Velazhahan R: Detoxification of oxalic acid by Pseudomonas fluorescens strain PfMDU2: Implications for the biological control of rice sheath blight caused by Rhizoctonia solani. Microbiol Res. 2005, 160: 291-298. 10.1016/j.micres.2005.02.002.View ArticleGoogle Scholar
- Nandakumar R, Babu S, Viswanathan R, Raguchander T, Samiyappan R: Induction of systemic resistance in rice against sheath blight disease by Pseudomonas fluorescens. Soil Biol Biochem. 2001, 33: 603-612. 10.1016/S0038-0717(00)00202-9.View ArticleGoogle Scholar
- Jayaraj J, Yi H, Liang GH, Muthukrishnan S, Velazhahan R: Foliar application of Bacillus subtilis AUBS1 reduces sheath blight and triggers defense mechanisms in rice. J Plant Dis Protect. 2004, 111: 115-125.Google Scholar
- Someya N, Nakajima M, Watanabe K, Hibi T, Akutsu K: Potential of Serratia marcescens strain B2 for biological control of rice sheath blight. Biocontrol Sci Technol. 2005, 15: 105-109. 10.1080/09583150400016092.View ArticleGoogle Scholar
- De Vleesschauwer D, Cornelis P, Hofte M: Redox-active pyocyanin secreted by Pseudomonas aeruginosa 7NSK2 triggers systemic resistance to Magnaporthe grisea but enhances Rhizoctonia solani susceptibility in rice. Mol Plant Microbe Interact. 2006, 19: 1406-1419. 10.1094/MPMI-19-1406.PubMedView ArticleGoogle Scholar
- Chernin L, Ismailov Z, Haran S, Chet I: Chitinolytic Enterobacter agglomerans antagonistic to fungal plant pathogens. Appl Environ Microbiol. 1995, 61: 1720-1726.PubMedPubMed CentralGoogle Scholar
- Ovadis M, Liu XG, Gavriel S, Ismailov Z, Chet I, Chernin L: The global regulator genes from biocontrol strain Serratia plymuthica IC1270: Cloning, sequencing, and functional studies. J Bacteriol. 2004, 186: 4986-4993. 10.1128/JB.186.15.4986-4993.2004.PubMedPubMed CentralView ArticleGoogle Scholar
- Meziane H, Sluis Van der I, Van Loon LC, Hofte M, Bakker P: Determinants of Pseudomonas putida WCS358 involved in inducing systemic resistance in plants. Mol Plant Pathol. 2005, 6: 177-185. 10.1111/j.1364-3703.2005.00276.x.PubMedView ArticleGoogle Scholar
- De Vleesschauwer D, Hofte M: Using Serratia plymuthica to control fungal pathogens of plants. CAB Reviews: Perspectives in Agriculture, Veterinary Science, Nutrition and Natural resources. 2007, [http://www.cababstractsplus.org/cabreviews].Google Scholar
- Iswandi A, Bossier P, Vandenabeele J, Verstraete W: Effect of seed inoculation with the rhizopseudomonad strain 7NSK2 on the root microbiota of maize (Zea mays) and barley (Hordeum vulgare). Biol Fertil Soils. 1987, 3: 153-158. 10.1007/BF00255776.View ArticleGoogle Scholar
- King E, Ward M, Raney D: Two simple media for demonstration of pyocyanin and fluorescin. J Lab Clin Med. 1954, 44: 301-307.PubMedGoogle Scholar
- Thuan NTN, Bigirimana J, Roumen E, Straeten Van der D, Hofte M: Molecular and pathotype analysis of the rice blast fungus in North Vietnam. Eur J Plant Pathol. 2006, 114: 381-396. 10.1007/s10658-006-0002-8.View ArticleGoogle Scholar
- Taheri P, Gnanamanickam S, Hofte M: Characterization, genetic structure, and pathogenicity of Rhizoctonia spp. associated with rice sheath diseases in India. Phytopathol. 2007, 97: 373-383. 10.1094/PHYTO-97-3-0373.View ArticleGoogle Scholar
- Rodrigues FA, Vale FXR, Datnoff LE, Prabhu AS, Korndorfer GH: Effect of rice growth stages and silicon on sheath blight development. Phytopathol. 2003, 93: 256-261. 10.1094/PHYTO.2003.93.3.256.View ArticleGoogle Scholar
- Ahn P, Kim S, Kang S, Suh SC, Lee YH: Rice defense mechanisms against Cochliobolus miyabeanus and Magnaporthe grisea are distinct. Phytopathol. 2005, 95: 1248-1255. 10.1094/PHYTO-95-1248.View ArticleGoogle Scholar
- Koga H, Dohi K, Nakayachi O, Mori M: A novel inoculation method of Magnaporthe grisea for cytological observation of the infection process using intact leaf sheaths of rice plants. Physiol Mol Plant Pathol. 2004, 64: 67-72. 10.1016/j.pmpp.2004.07.002.View ArticleGoogle Scholar
- Thordal-Christensen H, Zhang ZG, Wei YD, Collinge DB: Subcellular localization of H2O2 in plants. H2O2 accumulation in papillae and hypersensitive response during the barley-powdery mildew interaction. Plant J. 1997, 11: 1187-1194. 10.1046/j.1365-313X.1997.11061187.x.View ArticleGoogle Scholar
- Caracuel-Rios Z, Talbot NJ: Cellular differentiation and host invasion by the rice blast fungus Magnaporthe grisea. Curr Opin Microbiol. 2007, 10: 339-345. 10.1016/j.mib.2007.05.019.PubMedView ArticleGoogle Scholar
- Kankanala P, Czymmek K, Valent B: Roles for rice membrane dynamics and plasmodesmata during biotrophic invasion by the blast fungus. Plant Cell. 2007, 19: 706-724. 10.1105/tpc.106.046300.PubMedPubMed CentralView ArticleGoogle Scholar
- Ou S: Rice diseases. Surrey: Commonwealth Mycological Institute; 1985.Google Scholar
- Berruyer R, Adreit H, Milazzo J, Gaillard S, Berger A, Dioh W, Lebrun MH, Tharreau D: Identification and fine mapping of Pi33, the rice resistance gene corresponding to the Magnaporthe grisea avirulence gene ACE1. Theor Appl Genet. 2003, 107: 1139-1147. 10.1007/s00122-003-1349-2.PubMedView ArticleGoogle Scholar
- Mackill DJ, Bonman JM: Inheritance of blast resistance in near-isogenic lines of rice. Phytopathol. 1992, 82: 746-749. 10.1094/Phyto-82-746.View ArticleGoogle Scholar
- Koga H: Hypersensitive death, autofluorescence, and ultrastructural changes in cells of leaf sheaths of susceptible and resistant near-isogenic lines of rice (Pi-z(T)) in relation to penetration and growth of Pyricularia oryzae. Can J Bot. 1994, 72: 1463-1477. 10.1139/b94-180.View ArticleGoogle Scholar
- Rodrigues FA, Jurick WM, Datnoff LE, Jones JB, Rollins JA: Silicon influences cytological and molecular events in compatible and incompatible rice-Magnaporthe grisea interactions. Physiol Mol Plant Pathol. 2005, 66: 144-159. 10.1016/j.pmpp.2005.06.002.View ArticleGoogle Scholar
- Ganesan V, Thomas G: Salicylic acid response in rice: influence of salicylic acid on H2O2 accumulation and oxidative stress. Plant Sci. 2001, 160: 1095-1106. 10.1016/S0168-9452(01)00327-2.PubMedView ArticleGoogle Scholar
- Kawasaki T, Koita H, Nakatsubo T, Hasegawa K, Wakabayashi K, Takahashi H, Urnemura K, Urnezawa T, Shimamoto K: Cinnamoyl-CoA reductase, a key enzyme in lignin biosynthesis, is an effector of small GTPase Rac in defense signaling in rice. Proc Nac Sci USA. 2006, 103: 230-235. 10.1073/pnas.0509875103.View ArticleGoogle Scholar
- Ono E, Wong HL, Kawasaki T, Hasegawa M, Kodama O, Shimamoto K: Essential role of the small GTPase Rac in disease resistance of rice. Proc Nac Sci USA. 2001, 98: 759-764. 10.1073/pnas.021273498.View ArticleGoogle Scholar
- Vergne E, Ballini E, Marques S, Mammar BS, Droc G, Gaillard S, Bourot S, DeRose R, Tharreau D, Notteghem JL, Lebrun MH, Morel JB: Early and specific gene expression triggered by rice resistance gene Pi33 in response to infection by ACE1 avirulent blast fungus. New Phytol. 2007, 174: 159-171. 10.1111/j.1469-8137.2007.01971.x.PubMedView ArticleGoogle Scholar
- Torres MA, Jones JDG, Dangl JL: Reactive oxygen species signaling in response to pathogens. Plant Physiol. 2006, 141: 373-378. 10.1104/pp.106.079467.PubMedPubMed CentralView ArticleGoogle Scholar
- Van Breusegem F, Dat JF: Reactive oxygen species in plant cell death. Plant Physiol. 2006, 141: 384-390. 10.1104/pp.106.078295.PubMedPubMed CentralView ArticleGoogle Scholar
- Orozco-Cardenas ML, Narvaez-Vasquez J, Ryan CA: Hydrogen peroxide acts as a second messenger for the induction of defense genes in tomato plants in response to wounding, systemin, and methyl jasmonate. Plant Cell. 2001, 13: 179-191. 10.1105/tpc.13.1.179.PubMedPubMed CentralView ArticleGoogle Scholar
- Alvarez ME, Pennell RI, Meijer PJ, Ishikawa A, Dixon RA, Lamb C: Reactive oxygen intermediates mediate a systemic signal network in the establishment of plant immunity. Cell. 1998, 92: 773-784. 10.1016/S0092-8674(00)81405-1.PubMedView ArticleGoogle Scholar
- Govrin EM, Levine A: The hypersensitive response facilitates plant infection by the necrotrophic pathogen. Curr Biol. 2000, 10 (13): 751-757. 10.1016/S0960-9822(00)00560-1.PubMedView ArticleGoogle Scholar
- Apel K, Hirt H: Reactive oxygen species: Metabolism, oxidative stress, and signal transduction. Annu Rev Plant Biol. 2004, 55: 373-399. 10.1146/annurev.arplant.55.031903.141701.PubMedView ArticleGoogle Scholar
- van Wees SCM, Luijendijk M, Smoorenburg I, van Loon LC, Pieterse CMJ: Rhizobacteria-mediated induced systemic resistance (ISR) in Arabidopsis is not associated with a direct effect on expression of known defense-related genes but stimulates the expression of the jasmonate-inducible gene Atvsp upon challenge. Plant Mol Biol. 1999, 41: 537-549. 10.1023/A:1006319216982.PubMedView ArticleGoogle Scholar
- Verhagen BWM, Glazebrook J, Zhu T, Chang HS, van Loon LC, Pieterse CMJ: The transcriptome of rhizobacteria-induced systemic resistance in Arabidopsis. Mol Plant Microbe Interact. 2004, 17: 895-908. 10.1094/MPMI.2004.17.8.895.PubMedView ArticleGoogle Scholar
- Ahn IP, Lee SW, Suh SC: Rhizobacteria-induced priming in Arabidopsis is dependent on ethylene, jasmonic acid, and NPR1. Mol Plant Microbe Interact. 2007, 20: 759-768. 10.1094/MPMI-20-7-0759.PubMedView ArticleGoogle Scholar
- Ahn IP, Park K, Kim CH: Rhizobacteria-induced resistance perturbs viral disease progress and triggers defense-related gene expression. Mol Cells. 2002, 13: 302-308.PubMedGoogle Scholar
- Benhamou N, Kloepper JW, QuadtHallman A, Tuzun S: Induction of defense-related ultrastructural modifications in pea root tissues inoculated with endophytic bacteria. Plant Physiol. 1996, 112: 919-929.PubMedPubMed CentralGoogle Scholar
- Kim MS, Kim YC, Cho BH: Gene expression analysis in cucumber leaves primed by root colonization with Pseudomonas chlororaphis O6 upon challenge-inoculation with Corynespora cassiicola. Plant Biol. 2004, 6: 105-108. 10.1055/s-2004-817803.PubMedView ArticleGoogle Scholar
- Tjamos SE, Flemetakis E, Paplomatas EJ, Katinakis P: Induction of resistance to Verticillium dahliae in Arabidopsis thaliana by the biocontrol agent K-165 and pathogenesis-related proteins gene expression. Mol Plant Microbe Interact. 2005, 18: 555-561. 10.1094/MPMI-18-0555.PubMedView ArticleGoogle Scholar
- Eulgem T: Regulation of the Arabidopsis defense transcriptome. Trends Plant Sci. 2005, 10: 71-78. 10.1016/j.tplants.2004.12.006.PubMedView ArticleGoogle Scholar
- Knoth C, Eulgem T: The oomycete response gene LURP1 is required for defense against Hyaloperonospora parasitica in Arabidopsis thaliana. Plant J. 2008.Google Scholar
- Tsuda K, Sato M, Glazebrook J, Cohen JD, Katagiri F: Interplay between MAMP-triggered and SA-mediated defense responses. Plant J. 2008, 53: 763-775. 10.1111/j.1365-313X.2007.03369.x.PubMedView ArticleGoogle Scholar
- Navarro L, Zipfel C, Rowland O, Keller I, Robatzek S, Boller T, Jones JDG: The transcriptional innate immune response to flg22: interplay and overlap with Avr gene-dependent defense responses and bacterial pathogenesis. Plant Physiol. 2004, 135: 1113-1128. 10.1104/pp.103.036749.PubMedPubMed CentralView ArticleGoogle Scholar
- Bakker P, Pieterse CMJ, van Loon LC: Induced systemic resistance by fluorescent Pseudomonas spp. Phytopathol. 2007, 97: 239-243. 10.1094/PHYTO-97-2-0239.View ArticleGoogle Scholar
- Katagiri F: A global view of defense gene expression regulation – a highly interconnected signaling network. Curr Opin Plant Biol. 2004, 7: 506-511. 10.1016/j.pbi.2004.07.013.PubMedView ArticleGoogle Scholar
- Tao Y, Xie ZY, Chen WQ, Glazebrook J, Chang HS, Han B, Zhu T, Zou GZ, Katagiri F: Quantitative nature of Arabidopsis responses during compatible and incompatible interactions with the bacterial pathogen Pseudomonas syringae. Plant Cell. 2003, 15: 317-330. 10.1105/tpc.007591.PubMedPubMed CentralView ArticleGoogle Scholar
- Manandhar H, Lyngs Jorgensen H, Mathur S, Smedegaard-Petersen V: Induced resistance against blast. Advances in rice blast research. Edited by: Tharreau D. Rotterdam: Kluwer Academic Publishers, 2000, 2: 93-104. 2View ArticleGoogle Scholar
- Chen J, Zhang WD, Song FM, Zheng Z: Phospholipase C/diacylglycerol kinase-mediated signalling is required for benzothiadiazole-induced oxidative burst and hypersensitive cell death in rice suspension-cultured cells. Protoplasma. 2007, 230: 13-21. 10.1007/s00709-006-0195-x.PubMedView ArticleGoogle Scholar
- Nakashita H, Yasuda M, Nitta T, Asami T, Fujioka S, Arai Y, Sekimata K, Takatsuto S, Yamaguchi I, Yoshida S: Brassinosteroid functions in a broad range of disease resistance in tobacco and rice. Plant J. 2003, 33: 887-898. 10.1046/j.1365-313X.2003.01675.x.PubMedView ArticleGoogle Scholar
- Taheri P: Rhizoctonia sheath diseases on rice: characterization, genetic structure, and control by riboflavin-induced resistance. Phd. Thesis. Ghent University, Crop Protection Department; 2007.Google Scholar
- De Vleesschauwer D, Djavaheri M, Bakker PAHM, Hofte M: Pseudomonas fluorescens WCS374r-induced systemic resistance in rice against Magnaporthe oryzae is based on pseudobactin-mediated priming for a salicylic acid-repressible multifaceted defense response. Plant Physiology. 2008, 148: 1996-2012. 10.1104/pp.108.127878.PubMedPubMed CentralView ArticleGoogle Scholar
- Levine A, Tenhaken R, Dixon R, Lamb C: H2O2 from the oxidative burst orchestrates the plant hypersensitive disease resistance response. Cell. 1994, 79: 583-593. 10.1016/0092-8674(94)90544-4.PubMedView ArticleGoogle Scholar
- Govrin EM, Rachmilevitch S, Tiwari BS, Soloman M, Levine A: An elicitor from Botrytis cinerea induces the hypersensitive response in Arabidopsis thaliana and other plants and promotes the gray mold disease. Phytopathol. 2006, 96: 299-307. 10.1094/PHYTO-96-0299.View ArticleGoogle Scholar
- Kumar J, Huckelhoven R, Beckhove U, Nagarajan S, Kogel KH: A compromised Mlo pathway affects the response of barley to the necrotrophic fungus Bipolaris sorokiniana (Teleomorph : Cochliobolus sativus) and its toxins. Phytopathol. 2001, 91: 127-133. 10.1094/PHYTO.2001.91.2.127.View ArticleGoogle Scholar
- Mur LAJ, Kenton P, Draper J: In planta measurements of oxidative bursts elicited by avirulent and virulent bacterial pathogens suggests that H2O2 is insufficient to elicit cell death in tobacco. Plant Cell Environ. 2005, 28: 548-561. 10.1111/j.1365-3040.2005.01301.x.View ArticleGoogle Scholar
- Zago E, Morsa S, Dat JF, Alard P, Ferrarini A, Inze D, Delledonne M, Van Breusegem F: Nitric oxide- and hydrogen peroxide-responsive gene regulation during cell death induction in tobacco. Plant Physiol. 2006, 141: 404-411. 10.1104/pp.106.078444.PubMedPubMed CentralView ArticleGoogle Scholar
- Zaninotto F, La Camera S, Polverari A, Delledonne M: Cross talk between reactive nitrogen and oxygen species during the hypersensitive disease resistance response. Plant Physiol. 2006, 141: 379-383. 10.1104/pp.106.078857.PubMedPubMed CentralView ArticleGoogle Scholar
- Delledonne M, Zeier J, Marocco A, Lamb C: Signal interactions between nitric oxide and reactive oxygen intermediates in the plant hypersensitive disease resistance response. Proc Nac Sci USA. 2001, 98: 13454-13459. 10.1073/pnas.231178298.View ArticleGoogle Scholar
- Kachroo A, He ZH, Patkar R, Zhu Q, Zhong JP, Li DB, Ronald P, Lamb C, Chattoo BB: Induction of H2 O2 in transgenic rice leads to cell death and enhanced resistance to both bacterial and fungal pathogens. Transgenic Res. 2003, 12: 577-586. 10.1023/A:1025896513472.PubMedView ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.