Figure 3

Examples of MSAP profiles showing the tissue culture-induced alteration in cytosine methylation at the CCGG sites of random genomic loci in the three rice ssp. indica cultivars. (a), (b) and (c) are profiles of cultivars V14, V27 and R09, respectively. The labeling V14D, V27D and R09D are the donor seed-plants of the three genotypes; V14Ca1–2, V27Ca1–2 and R09Ca1–2 are calli; and, V14Reg1–5, V27Reg1–5 and R09Reg1–5 are regenerated plants. The four types of alteration in cytosine methylation pattern at the CCGG sites are indicated as A1 – CG hypomethylation, A2 – CG hypermethylation, B1 – CHG hypomethylation, and B2 – CHG hypermethylation. The primer combinations are indicated at bottom of the profiles.