Figure 8

Identification of histone modifications in histone H3 and H4 by Western Blotting. Ten μg soybean core histone mixtures were separated in 15% SDS-PAGE gel, and transferred to a PVDF membrane (one μg samples were used when antibodies that recognized H3K18Ac and H3K23Ac were used). A. Western blotting showed the presence of H3K18Ac, H3K23Ac, H3K4Tri-me, H3K27Tri-me, H3K36Tri-me, H3K79Mono-me and H3K79Di-me in histone H3. B. Western blotting showed the presence of H4K8Ac and H4K12Ac in histone H4. C. Coomassie stained SDS-PAGE gel showed the soybean core histone H2A, H2B, H3 and H4. Specific antibodies used were marked under their corresponding figure. Ac: acetylation; Me: methylation.