Genes | Ct valuesa |
---|
Gmpgip3
| 19.83 ± 1.00 |
Gmpgip5
| 32.68 ± 0.94 |
Gmpgip7
| 24.65 ± 0.88 |
GmELF1A
| 17.13 ± 1.01 |
- aTranscript levels were determined by quantitative RT-PCR. Gmpgip and GmELF1A (housekeeping) genes showed similar amplification efficiencies. Gmpgip3 crossed the detection of threshold 6 and 13 cycles before Gmpgip7 and Gmpgip5 transcripts, respectively. Means of Ct values are based on three technical replicates each of two biological replicates.