Figure 4

The cre1-1 mutation does not affect phosphate dependent regulation of miR171h as well as NSP2 transcript abundance and has no effect on mycorrhizal induced expression of NSP2 and arbuscular mycorrhizal marker genes. The relative expression of mature miR171h (A) or NSP2 (B) in roots of either WT plants (grey bars) or cre1-1 plants (white bars) determined at different phosphate fertilization and mycorrhizal conditions. -P: 20 μM phosphate, +P: 1 mM phosphate, −M: non-mycorrhizal roots, +M: mycorrhizal roots. (C) The relative expression of mycorrhizal the marker genes MtPt4 and RiTEF were determined in root material from the same plants shown in A and B (−P + M). All plants were harvested 4 wpi. Normalization of the expression data was carried out against a reference gene index (MtPdf2 and MtEf1). Data shown are average values of 3–6 biological replicates. Error bars indicate the standard errors. Different letters indicate statistical different values (P < 0.05). A and B: two-way ANOVA with Holm-Sidak multiple comparison. C: one-way ANOVA).