Figure 1
rga28 and gai-t6 mutant seeds show different germination phenotypes. Germination rates of wild-type, rga28, gai-t6 and gai-t6rga28 mutant seeds. Seeds were germinated with or without stratification. 28 Days After Ripening (DAR) seeds germinated respectively: under phyB-dependent germination conditions (A), white light, with or without stratification (B, C) and in the dark (D). White bars/symbols refer to seeds germinated with stratification, black bars/symbols without stratification. (E) Germination of seeds after 0, 7, 14, 21 DAR in white light, without stratification. The diagram at top depicts the light treatment scheme for the experiments. FRp, Far Red pulse; Rp, Red pulse of 90 μmol m−2 s−1; WLp, White Light pulse. Error bars = SEM. P values were obtained from a Student’s unpaired two-tail t test comparing the mutant with its control (* = p ≤ 0,05 ** = p ≤ 0,01).