Figure 8
Transient expression of the fla Lso triggered changes in the expression of genes related to plant defense in N. benthamiana . N. benthamiana leaves were inoculated with A. tumefaciens GV3101 containing pBin:fla Lso and GV3101 containing empty vector pBin as a control. The infiltrated spots were harvested at 2, 3, 4 and 8 days post-infiltration (dpi) for RNA isolation and cDNA preparation. RT-qPCR was performed to check gene expression of NbFLS2, NbWIPK, NbSIPK, NbBAK1, NbRbohB, NbCYP71D20, NbACRE31, NbACRE32, NbPlastocyanin and NbSerk3. The samples were normalized against NbEF1α. Data were shown as average fold gene induction in response to vector infiltrated samples from three independent experiments.