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Figure 2 | BMC Plant Biology

Figure 2

From: Functional analysis of the Landsberg erecta allele of FRIGIDA

Figure 2

Characterization of transgenic lines carrying the FRI-Col or FRI-Ler allele. (a) Flowering time expressed as total leaf number (rosette + cauline) from homozygous T3 single insertion lines containing a FRI-Col transgene are shown in comparison to the untransformed Col-0 wild type. (b) Experiment as in (a) but using homozygous T3 single insertion lines transformed with FRI-Ler. (c) Expression of FLC for the same genotypes as above. In this experiment, leaf tissue was collected from 10 day-old seedlings grown in long day conditions. The number in each bar indicates the number of biological replicates used. Expression was normalized to the expression of PP2A. (d) Flowering time after vernalization quantified as total leaf number for the same lines as above. Plants were vernalized for four weeks at 4°C and subsequently grown under long day conditions. The number in each bar indicates the number of individual plants per line analyzed. Error bars represent the standard error of the mean. Letters in each bar represent significance groups as determined by Tukey HSD test.

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