Figure 7

Effects of inhibiting auxin transport by NPA on in vitro embryogenesis initiation and progression. Embryogenic masses originated by secondary embryogenesis from immature zygotic embryos. A, B: Control cultures, without NPA, at the beginning of the treatment (A) and 20 days afterwards (B), when embryo development and new embryos (arrows) were observed. C, D: Culture containing 40 μM NPA at the beginning of the treatment (C) and 20 days afterwards (D), no further growth or new embryos were observed. Bars 5 mm.