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Figure 8 | BMC Plant Biology

Figure 8

From: Early markers are present in both embryogenesis pathways from microspores and immature zygotic embryos in cork oak, Quercus suberL

Figure 8

Immunolocalization of 5mdC, esterified (JIM7) and non-esterified (JIM5) pectins, and IAA in other systems. A-E: Zygotic torpedo embryo of Brassica napus. F-K: Mature anther of Quercus suber. A, F: Panoramic views of zygotic torpedo embryo and mature anther, resin sections stained by toluidine blue; squares in A and F indicate representative regions of proliferating embryo cells (A) and differentiated cells of anther wall (B) where the analysis is focused. B-E, G-K: Higher magnifications of immunofluorescence signals (green) for 5mdC (B, G), JIM5 (C, H), JIM7 (D, J) and IAA (E, K) antibodies. In B, DAPI staining for nuclei (blue) is merged with 5mdC immunofluorescence (green). Insets in B and G show representative nuclei of each cell types, at high magnification. Bars: A, F: 50 μm, B-E, G-K: 25 μm, insets: 5 μm.

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