Figure 8From: Plant disease resistance is augmented in uzu barley lines modified in the brassinosteroid receptor BRI1Transcript levels of BRI1 downstream and brassinosteroid metabolic genes in epibrassinolide (epiBL) treated seedlings of barley cultivars Akashinriki, Bowman and their uzu derivatives (Aka-uzu and Bow-uzu), as determined by quantitative RT-PCR analysis. Germinating seedlings were treated with Hoaglands solution containing 5 μm of the brassinazole for 4 days and then with Hoaglands solution plus or minus 0.2 μm epiBL. Gene expression was quantified in samples harvested either 12 or 24 h post-treatment. Gene represented are (A) PHYB activation tagged suppressor 1 protein, BAS1 (Contig3160), (B) XET (Contig5258) and (C) ROT3 (Contig12042). Bars indicate SEM (LSD0.05 A = 0.0021, B = 0.016, C = 0.0021).Back to article page