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Figure 1 | BMC Plant Biology

Figure 1

From: The "one-step" Bean pod mottle virus (BPMV)-derived vector is a functional genomics tool for efficient overexpression of heterologous protein, virus-induced gene silencing and genetic mapping of BPMV R-gene in common bean (Phaseolus vulgaris L.)

Figure 1

Bean pod mottle virus (BPMV)-induced expression of the green fluorescent protein ( GFP ) gene in leaves of P. vulgaris cv. Black Valentine after rub-inoculation with either infectious-DNA plasmids or leaf sap. (A-C) GFP fluorescence in the primary-inoculated leaf (A), in the primary and in third trifoliate leaf (B), and in the third trifoliate (BPMV-GFP/DNA plasmids) or fourth trifoliate leaf (BPMV-GFP/leaf sap) (C) at nine, 17 and 21 days post-inoculation (dpi), respectively. Leaves of plants inoculated with mock buffer, BPMV empty vector (BPMV-0) or GFP-expressing vector (BPMV-GFP) were visualized under natural light (top panel) and UV light (bottom panel) and photographed. Similar results were obtained from three independent experiments.

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BMC Plant Biology

ISSN: 1471-2229

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