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Table 2 Infection rates obtained after rub-inoculation with various quantities of RNA1- and RNA2-derived plasmids

From: The "one-step" Bean pod mottle virus (BPMV)-derived vector is a functional genomics tool for efficient overexpression of heterologous protein, virus-induced gene silencing and genetic mapping of BPMV R-gene in common bean (Phaseolus vulgaris L.)

Viral vector

Quantity of RNA1- and RNA2-derived plasmids (μg)

Infection ratea

BPMV-0

(1.5 + 1.5)

17% (1/6)

 

(3 + 3)

33% (2/6)

 

(5 + 5)

92-100% (11/12, 12/12)

BPMV-GFP

(5 + 5)

55% (5/9)

BPMV-GmPDS

(5 + 5)

33% (4/12)

BPMV-PvPDS-52 bp

(5 + 5)

50% (6/12)

BPMV-PvPDS-132 bp

(5 + 5)

67% (8/12)

BPMV-PvPDS-262 bp

(5 + 5)

67% (8/12)

BPMV-PvPDS-391 bp

(5 + 5)

58-91% (7/12,10/11)

  1. anumber of infected plants at 28 days post-inoculation/total of plants inoculated.