Table 3 PCR primers used for construction of the BPMV VIGS vectors and RT-PCR analyses
Name of primer | DNA sequence of primer (5′-3′) | Size of PCR product (bp) |
|---|---|---|
PvaPDS-F1 | cgggatccAGCAGAAGTCCCCTTCTGAG | 391 |
PvaPDS-R1 | cgggatccCTTGTGCACACAGCTTCC | |
PvaPDS-262fwd | cgggatccAGAATGGATTTCACGTAGT | 262 |
PvaPDS-262rev | cgggatccAACAGCACCTTCCATTGAAGCT | |
PvaPDS132-rev | cgggatccGGTGTTTTAACAACATGGTAC | 132a |
PvaPDS52-fwd | GATCGCTTTGCTTTGGTCTGCAGAAATTTCATCAGGAAAGAGTTTGGCAAGCTCAGT | - |
PvaPDS52-rev | GATCACTGAGCTTGCCAAACTCTTTCCTGATGAAATTTCTGCAGACCAAAGCAAAGC | - |
RNA1-fwd | CAAGCCCAAAGTGCTGAAGT | 160 |
RNA1-rev | GCAAATCCAACTGCCATTCT | |
RNA2-fwd | ATACCCCTAATGGCACAGGA | 269 |
RNA2-rev | GGAAATGTAACCACCCGAAT | |
RNA2-BamH1-fwd | TGACAATCCCAAACAGTCTACAG | 234 |
RNA2-BamH1-rev | AGCATACTCAACGAGAGGGTCA | |
RNA2-GFP-fwd | TGAGGTTCAGGCTCAGATGGA | 863 |
RNA2-GFP-rev | GGCCTGGATTTGATTCTACA | |
PvPDS-fwd | ACATCTTCTTTGGGGCTTACC | 200 |
PvPDS-rev | CAGCATCTCGTTGTTCCTCA | |
PvUBI-fwd | CAGCTGGAGGATGGAAGG | 200 |
PvUBI-rev | TCCGAACTCTCCACCTCAAGA |