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Table 4 Transformation frequencies using axillary buds from in vitro grown crambe plants

From: Regeneration and transformation of Crambe abyssinica

Experiment ID

Selection

Vector

Frequency

Tr11-05

Consistent 10 mg · L-1

pBinGlyRed (AscI)

1.6%

Tr12-01

Shifting 10 → 25 → 10 mg · L-1

pJS-M14

0.5%

Shifting 10 → 20 → 10 mg · L-1

2.0%

  1. Note: the success of transformation was verified by PCR, GUS staining or fluorescence. Selection is on kanamycin.
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BMC Plant Biology

ISSN: 1471-2229

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