Table 5 Transformation frequencies using different vectors in two Agrobacterium strains and crambe cotyledonary node explants with the optimized protocol
Experiment code | Vector | Vector size/bp | A.tum | Period/week | Percentage of transformation |
|---|---|---|---|---|---|
Tr08-01 | pJS-M14 | 21,071 | AGL0 | 20 | 9.9% |
Tr08-02 | pJS-M14 | 21,071 | AGL0 | 19 | 4.4% |
Tr12-01 | pJS-M14 | 21,071 | AGL0 | 20 | 4.0% |
T11-03 | pJS-M14 | 21,071 | AGL0 | 19 | 7.5% |
T10-01 | pHan2 | 16,783 | AGL0 | 24 | 1.5% |
T09-10 | pBinGlyRed (Asc I) | 14,937 | AGL1 | 19 | 4.0% |
T10-02 | pBinGlyRed (Asc I) | 14,937 | AGL1 | 16 | 3.8% |
T10-03 | pBinGlyRed (Asc I) | 14,937 | AGL1 | 18 | 5.8% |
T09-10 | pBinGlyRed (Kpn I) | 17,662 | AGL1 | 19 | 1.0% |
T10-04 | pBinGlyRed (Kpn I) | 17,662 | AGL1 | 18 | 2.0% |
T12-02 | pHellsgate LPAT2-RNAi | 15,392 | AGL1 | 20 | 2.5% |
Tr12-05 | pWatergate-3G | 23,746 | AGL1 | 20 | 2.3% |