Figure 1

RfBP expression and flavin content in leaves of the wild-type (WT) plant and RfBP -expressing (RfBP⁺) or RfBP -silencing (RfBP^-) line of Arabidopsis. Plants were grown for 10 days in long days (16 hour light) or 25 days in short days (8 hour) before use in the following analyses. (a) Northern blotting with the probe specific to the RfBP gene or the constitutively expressed EF1 α gene used as a reference. (b) Analysis of plant proteins by the gel electrophoresis. Protein bands were visualized by gel staining with Coomassie G-250. Molecular makers are indicated. (c) Quantification of flavin concentrations. Data shown are mean values ± standard deviation bars of results from three independent experiments each containing three repeats and 15 plants per repeat. Different letter on bar graphs indicate significant differences by analysis of variance and least significant difference test (P < 0.01).