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Figure 4 | BMC Plant Biology

Figure 4

From: Production of superoxide from Photosystem II in a rice (Oryza sativaL.) mutant lacking PsbS

Figure 4

Time course for generation of individual ROS in thylakoids of PsbS-KO and wild-type (WT) rice under photoinhibitory illumination at room temperature. (a) Singlet oxygen production was monitored as relative increasing of SOSG (10 μM) fluorescence at 530 nm. (b) Fluorescence emission of dihydroethidium (25 μM) at 590 nm was used to detect superoxide production. (c) Fluorescence emission of DCFDA (10 μM) at 525 nm was used to detect hydrogen peroxide. Thylakoid suspensions were illuminated at 700 μmol photons m-2 s-1. Samples contained 10 μg chlorophyll per mL. Each point represents mean of at least 4 experiments (SD indicated by bar; in some cases, SD is less than marker size) and the asterisks denote the results that were significantly different from those in the wild type (*P < 0.05). The statistical significance was evaluated using the Student's t -test.

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