Figure 1From: A rapid live-cell ELISA for characterizing antibodies against cell surface antigens of Chlamydomonas reinhardtii and its use in isolating algae from natural environments with related cell wall componentsCassettes for over-expression in E. coli of the V H H B11 gene encoding an antibody that recognizes a specific C. reinhardtii cell surface antigen. A. VHH B11 cassette for expression of the VHH B11 fusion protein containing the Trx A protein at the N-terminus, an internal 6 × His tag, and an E-tag epitope at the C-terminus. B. GFP-VHH B11 cassette: identical to VHH B11 cassette except for insertion of a GFP or mCherry coding region immediately upstream and in-frame with the VHH coding region.Back to article page