Figure 5

Co-transfection analysis in At7 protoplasts indicate in vivo regulatory potential of Bv_iogq on A. thaliana and B.vulgaris CHS and FLS promoters. Results from co-transfection experiments in A. thaliana protoplasts. Promoter fragments of the (A) A. thaliana AtCHS, AtFLS1 and AtDFR genes, and (B) B. vulgaris BvCHS_phae and BvFLS_ckiq genes (reporters) were assayed for their responsiveness to the effectors AtMYB12/PFG1, AtMYB123/TT2 and Bv_iogq expressed under the control of the CaMV 35S promoter. Subscripted numbers indicate promoter fragment length. The figure shows mean GUS activity resulting from the influence of tested effector proteins on different reporters. Data from a set of four replicates are presented.