Figure 8
The effect of selenite on glutathione metabolism. The levels of glutathione were measured enzymatically in total root tissue (a) and root tips (b) 8–12 mm in length from untreated plants and plants treated with 50 μM selenite for 1 and 3 d. Shown are the mean and SE from 4 different plants, and represent 2 other biological experiments. Lowercase letters represent significant differences among treatments (p < 0.05). (c) Cell viability and glutathione content in root tips were estimated as the fluorescence of fluorescein diacetate (left) and monochlorobimane (right), respectively from 8–10 pooled root tips from 4 plants per treatment on day 3. (d). Abundance of the putative GGCT, APR, and SiR polypeptides were estimated by loading 20 μg of denature protein from root tissue per lane on SDS-PAGE and analyzed by immunoblotting. The immunoblot is representative of at least three biological experiments, and numbers below each blot represent the mean pixel intensity of each immunoreactive band relative to control on day 0. Asterisks indicate a significant difference in band intensity in selenite-treated plants compared to untreated plants (p < 0.05).