Table 2 Nucleotide sequences of primers specific to cyanidin-based anthocyanin biosynthetic genes and Actin1 gene used for qRT-PCR
Gene | Forward primer 5′-3′ | Reverse primer 5′-3′ |
|---|---|---|
PAL1 | ACATTACCCCTTGCTTGCCACT | TCAAAGAATCCACCATCAACTCC |
PAL3/PAL4 | CTGGCATGGCCTCTATGGTACT | TGTCCTGGATGGTGCTTCAACT |
CA4H1 | GTGGAGGCCAACGGAAATG | CGTCCGATTGTGATACCGAGA |
CA4H2 | CCGGCAAAGAGCAAAGTTGT | CAGCCCCGAATGGTAGGAAT |
4CL1 | AAACACCTGCCGTTACACTCG | CGGAAGCAAGATCATCATCGTAT |
4CL2 | AGAGCCAAGTTTCCTAATGCCA | TCCCCGTTGATTCCTTGGTAG |
CHS1 | TTCCACCTTCTCAAAGATGTTCC | GCTCAACTCTGTTTCAACTTGGTC |
CHS2/CHS9 | ATCAGGAAAAGGCAGAGGGC | ATCCGCCTGGTAGACGCAGT |
CHI1 | TCCTGCCACGGTCAAACCT | AAGAGCGAGCGACGGAATC |
F3H1 | GAGTACAGTGAGAAGCTGATGGGTC | GGTTGAGGGCACTTGGGATAG |
F3′H1 | TTGAGGATGGTGAAGGTGGGA | CTTTTGGGCGACGCAGAAC |
DFR1 | GTTATCAAGCCTACCGTACAGGG | AGTTCCAGCAGACGAAGTGTAAAT |
LDOX1/LDOX2 | AGGTGCCCACAGTCGACATAGC | CGCCTGTCCAGCCACTCTAA |
DcActin1 | CGGTATTGTGTTGGACTCTGGTGAT | CAGCAAGGTCAAGACGGAGTATGG |