Skip to main content
Figure 9 | BMC Plant Biology

Figure 9

From: Accumulation and cellular toxicity of aluminum in seedling of Pinus massoniana

Figure 9

Effects of different concentrations of Al on the translocation of C23. Simultaneous location of C23 after the reaction with primary anti-C23 antibody and secondary antibody conjugated with TRITC (red) and DNA after the reaction with DAPI (blue) in the same single optical section obtained with the confocal scanning laser microscopy. A1-D1, C23 detection; A2-D2, DNA detection; A3-D3, Merged image; A4-D4, Bright-field image of the cells. A1-A3, Showing that C23 was distributed in nucleolus in control cells. B-C, Showing that C23 was migrated from nucleolus to nucleoplasm and cytoplasm in the cells exposed to10-4 M Al for 72 h. D1-D3, Showing that in some cells bigger fluorescence signals of C23 appeared and were extruded from nucleolus into cytoplasm after the exposure of 10-2 M Al for 72 h. Scale bar =10 μm.

Back to article page