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Table 2 Primers used for modified 5′ RLM-RACE mapping of the miRNA cleavage sites and putative target protein

From: Identification and characterization of cold-responsive microRNAs in tea plant (Camellia sinensis) and their targets using high-throughput sequencing and degradome analysis

miRNAs

Targets gene

Putative target protein

Conserved gene inA. thaliana(E-scroe)

Gene-specific primer

Nested gene-specific primer

csn-miR319b-1

CV014890.1

Unknown protein

 

CCACGCTGGGCACTGTATGATGAT

 

csn-miR396b-2

JK476458.1

Unknown protein

 

ATTCCCGCCAACAGCATCAATGTC

CACGCATCACCAAACACAGCGATAAG

csn-miR396c

FS943373.1

Plant calmodulin-binding protein-related

AT5G07820.1(5E-06)

AACGCTTTCCACCACCACCTCCAAG

CGCAGTCACCTCGGCTTTCTTAGC

csn-miR398

FS954022.1

Rubredoxin-like superfamily protein

AT1G80230.1(5E-34)

CTTCACCTCCAGGGCATCCAACAAT

GTCCCGTGGCAATAGGCATCACATCT

csn-miR408

GD254786.1

Transposable element gene

AT5G29056.1(4E-07)

GCCAGGGAGAGAGCAAATGAAGAAGTTC

CCAGCCTTGTTCACACTGACCACATTGT

csn-miR408

FS955921.1

VQ motif-containing protein

AT1G28280.1(1E-12)

GCCAGGGAGAGAGCAAATGAAGAAGTTC

CCAGCCTTGTTCACACTGACCACATTGT