Figure 6

Silique elongation and expressional analyses of transgenes in the At80MP-LV and At80MD Arabidopsis. A. A schematic representation of the At80MP-LV and At80MD truncated constructs. The letters indicate amino acids at the beginning and end of domains. B. The At80MP-LV transgene was unable to rescue the atmyb80 homozygous mutant and the plant remained completely male sterile (line 6). C. Plant fertility was reduced in the heterozygous atmyb80 mutant transformed with the At80MP-LV transgene (line 8). D. The partially male sterile phenotype of the wild-type Arabidopsis transformed with the At80MD construct (line 13). E. The expression of At80MP-LV was detected in the homozygous atmyb80 mutants (line 6 and 11, 0% fertility) and the heterozygous atmyb80 mutants (line 4, 7, 8 and 13, 5% to 95% fertility). F. At80MD transcript levels and plant fertility were determined in the selected lines. The expression levels of endogenous AtMYB80 were reduced in all lines. Wild type (WT) is the negative control. Error bar represents SD.