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Figure 8 | BMC Plant Biology

Figure 8

From: MYB80 homologues in Arabidopsis, cotton and Brassica: regulation and functional conservation in tapetal and pollen development

Figure 8

Phenotype of silique elongation and expressional analyses of transgenes in the P Gh80 :Gh80-32R2 and P Gh80 :Gh80MD-32R2 Arabidopsis. A. A schematic representation of the P Gh80 :Gh80-32R2 and P Gh80 :Gh80MD-32R2 chimeric constructs. B and C. Wild-type Arabidopsis possessing either the P Gh80 :Gh80-32R2 (B) or P Gh80 :Gh80MD-32R2 (C) transgene has a partially sterile phenotype. D and E. Alexander's staining shows the majority of pollen grains lack cytoplasm and are aborted in the P Gh80 :Gh80-32R2 (D) and P Gh80 :Gh80MD-32R2 (E) anthers. Scale bar = 85 μm. F and G. The relative expression levels of the P Gh80 :Gh80-32R2 (F) and P Gh80 :Gh80MD-32R2 (G) transgenes in the selected lines. The higher the ratio (P Gh80 :Gh80-32R2 or P Gh80 :Gh80MD-32R2 vs. AtMYB80), the lower the plant fertility obtained. Gh32R2, P Gh80 :Gh80-32R2; GhMD32R2, P Gh80 :Gh80MD-32R2; Endo80, endogenous AtMYB80. Error bar represents SD.

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BMC Plant Biology

ISSN: 1471-2229

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