Figure 2

Transcript levels of endogenous GSTF8 and transgenes in WT^LUCand hsi2 mutants carrying either Kan^Ror Kan^Stransgene locus or both. Real-time reverse transcription quantitative PCR was used to determine the relative transcript levels of endogenous GSTF8, LUC and NPTII genes in five day old seedlings of various genotypes. GSTF8 produces two different transcripts with different fragment lengths by alternative start sites namely GSTF8-Long and GSTF8-Short[34]. Expression of GSTF8-Total represents transcripts from both GSTF8-Long and GSTF8-Short versions whereas GSTF8-Long expression level corresponds to GSTF8-Long transcript. EF1α was used for normalization. Data represent means (’sD) of two biological replicates with three technical replicates each. Significant differences in LUC transcript levels between the three luciferase reporter lines in the wild-type background and the respective hsi2-4 mutant background, determined using two-tailed Student’s t-test assuming unequal variances, are indicated by letters (a = p < 0.001 and b = p < 0.0001).