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Figure 2 | BMC Plant Biology

Figure 2

From: Identification of a novel major locus for gray leaf spot resistance in Italian ryegrass (Lolium multiflorumLam.)

Figure 2

Comparative rice Chr 1 - Italian ryegrass LG 3 genetic maps and a LOD score plot obtained by QTL analysis. Markers on the rice Chr 1 genetic map were selected from the rice EST map [17] to develop markers with intron-scanning primers. The developed rice EST-derived markers are indicated by EST clone names (e.g., S14186) provided by the Rice Genome Project (http://rgp.dna.affrc.go.jp/E/Publicdata.html). AFLP markers are indicated by following the nomenclature of AFLP primer enzyme combinations of Key genes (e.g.. E32/M59). SSR markers are indicated by the names (e.g., 08-08B) given by Hirata et al. [14]. Comparative loci between rice and Italian ryegrass are shown in bold on the rice Chr 1 genetic map and are connected by solid lines. Locations of rice disease resistance gene loci on the rice Chr 1 reviewed by Ballini et al. [19] are shown in italics. Genetic distances are measured in centimorgans (cM) against the ruler on the left side of the figure. The linkage map for the ryegrass LG 3 was used for QTL analysis. The graph on the right side of the linkage map shows LOD score plots obtained by interval mapping. The light gray, gray, and black curves represent score plots for young leaves, expanded leaves, and total data obtained from four inoculation experiments, respectively. A broken vertical line indicates a LOD significance threshold level, 3.6, calculated by a permutation test (P < 0.05) with 1000 repetitions. The position of LmPi2 is shown with an inner and outer vertical bar for 1-LOD and 2-LOD support interval, respectively. The position of LmPi2 and the LOD significance threshold level were calculated based on a result of QTL analysis calculated with the total data obtained from four inoculation experiments.

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