Figure 4

Expression of omega-3 FAD genes in G. hirsutum seeds, petals and leaves. (A) Developing cottonseeds were harvested from G. hirsutum plants at the indicated times, then RNA-seq analysis was performed as described. Transcripts were quantified as “reads per kilobase per million mapped reads” (RPKM). For simplicity, data for A and D homoeologous sequences were combined. RNA-seq was also performed on cotton petals (B) as well as cotton leaves (C), as described [59],[60]. Values represent average and standard deviation of three biological replicates. For data presented in panels (B) and (C), student’s t-test was used for comparison of FAD7/8-1 to FAD7/8-2, and * denotes p <0.05.