Figure 5
From: In vivo functional analysis of a nuclear restorer PPR protein
ORF138 expression in CMS, fully restored and partially restored plants. (A) Mitochondria were isolated from petals (Pe), anthers (An), styles (St), sepals (Se) and leaves (Le) of CMS and fully fertility restored Rfo transgenic plants. 5 μg of mitochondrial protein from each organ was subjected to immunoblot analysis with anti-ORF138 antisera. (B) Mitochondria isolated from different organs of T0 Rfo? transgenic plants expressing different degrees of male sterility were subjected to SDS-PAGE and ORF138 was detected as in panel; Rfo?-22 is the most male-sterile of the recovered plants. Panels below the immunoblots show the protein on the membranes as detected with Ponceau red.