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Figure 1 | BMC Plant Biology

Figure 1

From: Evaluation and integration of functional annotation pipelines for newly sequenced organisms: the potato genome as a test case

Figure 1

Overview of pipeline comparison, validation of accuracy and integration processes. (A) The PGSC and ITAG gene models were used as input for the six pipelines assessed. (B) The annotation from each pipeline was transformed into gene ID – GO term associations. (C) Annotations were compared by the number of annotated gene models, the number of GO terms associated per gene model, and GO similarity. (D) The quality and comprehensiveness of the annotation of each pipeline were calculated by comparing their predictions to experimentally validated annotation (gold standard). In addition, gene co-expression data were used to test if genes predicted to share the same GO processes are significantly co-expressed. (E) An integrated annotation using the ensemble of results of all pipelines was created and validated using the same criteria in D. Results of the ensemble annotations were compared to those of the individual pipelines.

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