Figure 6

Phenotypic, molecular, and biochemical analyses of pBDX::BDX antisense transgenic lines. A) Representative wt and ASBDX plants, arrows indicate differences in siliques respect to wt. B) Representative open siliques from wt and ASBDX plants C) Close up from wt and ASBDX siliques. D) Longitudinal section of pistil from stage 13 flower from ASBDX. Scale bar =200 μm. A close-up of one ovule is shown. Scale bar =25 μm. E) Quantitative analysis of silique length in wt and ASBDX lines 1, 2, and 3. F) PME activity in total protein extracts from rosette leaves of wt and ASBDX lines 1, 2 and 3. For all lines, experiments were performed at least three times with different plants. Values shown are mean ± standard error. G) Expression of pBDX::BDX antisense transgene significantly decreased endogenous BDX transcript level in inflorescences of all three lines. Different letters indicate significant differences.