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Figure 1 | BMC Plant Biology

Figure 1

From: Trihelix transcription factor GT-4 mediates salt tolerance via interaction with TEM2 in Arabidopsis

Figure 1

GT-4 gene expression and protein localization and transcriptional activation ability. (a) GT-4 expression levels in response to salt stress. Bars indicate SD (n = 3). (b) GT-4 expression in various organs of Arabidopsis plants. Bars indicate SD (n = 3). (c) Subcellular localization of GT-4 protein in Arabidopsis protoplasts. (d) Effector constructs used in the Arabidopsis protoplast transient assay. Each effector contained a GAL4 DNA-binding domain (GAL4DBD). The GAL4DBD effector was used as a negative control, and effector VP16, was used as a positive control. Full length GT-4, GT-4-N (1–113) and GT-4-C (114–372) was fused with the GAL4DBD and expression was driven by the 35S promoter plus the translation enhancer Ω sequence. (e) Transcriptional activation ability of GT-4, GT-4-N and GT-4-C as revealed by relative LUC activity of the reporter. The effectors and the GAL4-LUC reporter were co-transfected. Bars indicate SD (n = 4).

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