Figure 8From: Genome-wide identification of mitogen-activated protein kinase gene family in Gossypium raimondii and the function of their corresponding orthologs in tetraploid cultivated cottonExpression patterns of three MAPK genes induced by V. dahliae and VIGS analysis. (a) Q-PCR analysis of the expression of three MAPK genes after inoculation by V. dahliae. The error bars were calculated based on three biological replicates using standard deviation. “*”: significant difference (P < 0.05); “**”: significant difference (p < 0.01). (b) Phenotypes after TRV-VIGS silencing of three MAPK genes and GhCLA1. After two weeks post-treatment with TRV1 and TRV2/TRV2-MPKs, the treated plants exhibited normal growth. TRV1- and TRV-GhCLA1-treated plants exhibited a photobleaching phenotype. (c) Gene expression of MPK9, MPK13 and MPK25 in silenced and control plant leaves by Q-PCR analysis; The error bars were calculated based on three biological replicates using standard deviation. “*”: significant difference (P < 0.05); “**”: significant difference (p < 0.01). The cotton histone 3 (AF026714) was used as the reference gene.Back to article page