Figure 1

Transgene transcripts normalized relative expression levels measured by delta-delta Cq method and Pffafl [ [19] correction equation. The epsps, cry1A.105 and cry2Ab2 transgenes were quantified from stacked versus single transgenic maize events grown under controlled conditions at V3 stage. Experiment 1 (A) and under the same conditions in Experiment 2 (B). Samples are means of three pools, each derived from five different plants. ‘RR’ samples are transgenic maize seedlings from MON-ØØ6Ø3-6 event, ‘Bt’ samples are from MON-89Ø34-3 event, and ‘RRxBt’ samples are transgenic maize seedlings from MON-89Ø34-3 x MON-ØØ6Ø3-6 event. Bars indicate standard deviation and statistically significant values (P < 0.05) are represented by ‘*’.