Figure 6From: Functional analysis of Flavonoid 3′,5′-hydroxylase from Tea plant (Camellia sinensis): critical role in the accumulation of catechinsOptimization of CsF3′5′H expression in Saccharomyces cerevisiae ‘WAT11’. (A) Comparison of amino acid sequences encoded by CsF3′5′H and VvF3′5′H proteins. The boxed regions represent fusion between CsF3′5′H and VvF3′5′H. (B) Primary structure schemes of expressed CsF3′5′H sequence variants and resulting expression strength expressed as CsF3′5′H activity · L−1 culture. Enzyme activity was expressed as pKat · L−1 culture. The data represent the mean ± SD from three independent measurements.Back to article page