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Table 1 Accepted substrates and enzyme activity units for F3′5′H

From: Functional analysis of Flavonoid 3′,5′-hydroxylase from Tea plant (Camellia sinensis): critical role in the accumulation of catechins

Substrate

Modified-CsF3′5′H (FSI)

VvF3′5′H

Class

3′-Hydroxyla-tion product (pKat · L −1)

5′-Hydroxylat-ion product (pKat · L −1)

3′-Hydroxylat-ion product (pKat · L −1)

5′-Hydroxylat-ion product (pKat · L −1)

Naringenin

12.79 ± 0.11

26.47 ± 1.08

24.27 ± 0.70

23.73 ± 0.85

Flavanone

Eriodictyol

 

0.13 ± 0.79

 

0.84 ± 0.13

Kaempferol

4.58 ± 0.39

8.54 ± 0.40

2.84 ± 0.76

5.53 ± 0.44

Flavonol

Quercetin

 

0.58 ± 0.27

 

0.36 ± 0.19

Dihydro-kaempferol

3.42 ± 0.54

2.69 ± 0.48

Dihydro-flavonol

Dihydro-quercetin

 

0.08 ± 0.03

 

0.05 ± 0.08

Pelargonidin

Antho-cyanin

Cyanidin

 

 

Catechin

 

 

Flavan-3-ols

Epicatechin

 

 

  1. Enzyme activity was expressed as pKat · L−1 culture. The data represent mean ± SD from three independent measurements. — indicates results below the detection limit.