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Table 2 Comparison of steady-state kinetic parameters for cinnamate 4-hydroxylation in yeast microsomes

From: Functional analysis of Flavonoid 3′,5′-hydroxylase from Tea plant (Camellia sinensis): critical role in the accumulation of catechins

Substrate

Modified-CsF3′5′H (FSI)

VvF3′5′H

Class

Km (μM)

Vmax (pM · min −1 ·mg −1Microsome)

Km (μM)

Vmax (pM ·min −1 ·mg −1Microsome)

Naringenin

3.22 ± 0.31

124.49 ± 10.11

2.03 ± 0.34

183.00 ± 11.02

Flavanone

Kaempferol

4.33 ± 0.19

306.00 ± 7.89

5.30 ± 0.71

327.00 ± 12.02

Flavonol

Dihydro-kaempferol

3.26 ± 0.25

219.00 ± 9.37

3.74 ± 0.54

220.18 ± 8.79

Dihydro-flavonol

  1. Experiments were carried out in 50 mM phosphate buffer pH 7.0 at 28°C for 30 min. Protein concentration of F3′5′H -transformed yeast microsomes was 0.1 mg/ml in the reaction system. The data represent the mean ± SD from three independent measurements.