Figure 2

AtRAD21.1 has an emergency-like transcription response to DNA dsb damage. Steady-state AtRAD21.1 and AtRAD51 transcript levels increase abruptly immediately after the end of irradiation exposure (AEI) in four weeks old Col rosette leaves irradiated with 316 Gy (2.65 Gy/minute; source: Co60); Non-irradiated samples were used as reference (i.e. 1 fold). The AtRAD21.1 and AtRAD51 steady-state transcript level peak is detected 1 to 2 hours (AEI) (60 to 120 minutes); peaks of circa 100-fold and 317-fold increase in AtRAD21.1 and AtRAD51, respectively. AtRAD21.1 steady-state transcript levels revert to normal expression levels after 48 hours (2880 minutes) AEI. AtRAD21.2 and AtRAD21.3 transcript levels variation is mild, in comparison to AtRAD21.1, even if AtRAD21.3 transcript steady-state levels increase by two-fold in response to DNA dsb. Values are the mean of three biological replicates for each time point. The relative transcript content was calculated using Actin2 and AtEF1αA4 as the reference genes, and normalized against the non-irradiated sample. The error bars represent the standard deviation. Quantitative RT-PCR data is available in Additional file 1: Table S2.