Figure 4
From: Identification and use of the sugarcane bacilliform virus enhancer in transgenic maize
Enhancer analysis of the SCBV-IM promoter. Two fragments of the SCBV-IM promoter were fused with the truncated ZmADH1 promoter and the luciferase reporter gene. These constructs were tested for activity in transient assays and compared with a construct containing only the minimal ZmADH1 promoter. These constructs were transfected into maize Hi-II cultures with a ZmUBI1::GUS construct. Activity is reported as the ratio of luciferase activity (LU) from the test construct to GUS activity (GU) from the internal control construct. Error bars represent the standard deviation of three measurements. Analysis of Variance and Tukey-Kramer HSD tests (α = 0.05) indicate significant differences in LU/GU ratios for all constructs tested.