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Figure 2 | BMC Plant Biology

Figure 2

From: Microbe-associated molecular pattern-induced calcium signaling requires the receptor-like cytoplasmic kinases, PBL1 and BIK1

Figure 2

Calcium elevation is reduced after treatment with flg22 (A), elf18 (B) and AtPep1 (C) but not with chitin octamer (ch8) (D) in all five cce5 mutants. To enable absolute cytosolic calcium measurements, the parental HVA1 (C24) line was transformed with the pMAQ2 construct (designated as HVA1-P) and used as a reference. The indicated genotypes were crossed with HVA1-P (i.e. a C24 Arabidopsis accession expressing cytosolic apoaequorin). Seedlings from the F2 populations were screened for the cce phenotype to identify homozygous cce5 plants, the mutations verified by CAPS marker analysis, and the progenies used for calcium measurements. Error bars represent standard errors of the mean.

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