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Figure 5 | BMC Plant Biology

Figure 5

From: Microbe-associated molecular pattern-induced calcium signaling requires the receptor-like cytoplasmic kinases, PBL1 and BIK1

Figure 5

MAMP/DAMP-induced calcium elevations in T-DNA insertion mutants of PBL1 , BIK1 , PBL2 and PBS1 . T-DNA insertion mutants of pbl1, bik1 or a pbl1bik1 double mutant were crossed with the pMAQ2 aequorin transgenic line. After verifying the mutant genotypes by PCR, changes in calcium levels induced by the indicated MAMPs were measured in the F2 or F3 generations (A). Due to silencing when crossed to the pMAQ2 reporter for the pbl2 (B) and pbs1 (C) mutants, crosses were made with an independently generated pUBQ-AEQ line with the expression of the apoaequorin reporter driven by the UBIQUITIN-10 promoter. Hence, the parental pUBQ-AEQ line in Col-0 background was used as a reference for the wild type calcium signature for pbl2 and pbs1. (Two independent pbl2 mutant alleles were included). Error bars represent standard errors.

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