Figure 10
The antagonistic effects of RfBP and NFXL1 gene deletion ( nfxl1 ) on flowering and related responses. Shown here are measurements on METC gene expression in leaves (a), leaf H2O2 concentrations (b), FD and AP1 expression at the shoot apex (c), flowering time (d), rosette leaf number (e). The antagonistic effects were analyzed on WT, RfBP+, nfxl1 mutant, and RfBP+nfxl1 hybrid plants. Gene expression was quantified by real-time RT-PCR using EF1α as a reference gene and H2O2 was measured by spectrometry, and both analyses were performed on 12-day-old plants. Data shown are mean values ± standard deviations based on three experimental repeats each containing 30 plants. On bar graphs, different letters in regular and italic fonts indicate significant differences by analysis of variance using Fisher’s least significant difference test and Tukey-Kramer’s test, respectively (n = 3; P < 0.01).