Figure 4

Expression levels of METC genes in riboflavin-fed and water-treated WT, RfBP ⁺, and RfBP ⁻plants. Water or an aqueous riboflavin solution was used to immerse seeds and treat 10-day-old plants by spraying over plant tops. Gene expression in the two youngest expanded leaves of 12-day-old plants was analyzed by real-time RT-PCR using EF1α as a reference gene. Ratios of transcript quantities between the tested METC genes and EF1α were quantified as mean values ± standard deviations from six experimental repeats each containing 15 plants. On bar graphs, different letters in regular and italic fonts indicate significant differences by analysis of variance using Fisher’s least significant difference test and Tukey-Kramer’s test, respectively (n = 6; P < 0.01).