Figure 7

The effects of riboflavin feeding treatment on flowering and expression of FD and AP1 . An aqueous solution of riboflavin or water was used to immerse seeds and treat 10-day-old plants by spraying over plant tops. In (a), flowering time and rosette leaf number were scored. In (b), relative levels of FD and AP1 expression in shoot apices of 12-day-old plants were quantified by real-time RT-PCR using the constitutively expressed EF1α gene as a reference. Data shown are average values ± standard deviations based on three experimental repeats each containing 15 plants. On bar graphs, different letters in regular and italic fonts indicate significant differences by analysis of variance using Fisher’s least significant difference test and Tukey-Kramer’s test, respectively (n = 3; P < 0.01).