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Table 1 Differential protein spots between WT and MT anthers identified by MALDI-TOF-MS

From: Proteomic analysis of anthers from wild-type and photosensitive genetic male sterile mutant cotton (Gossypium hirsutum L.)

Sp. No a

Protein ID b

Description c

Sco. d

The. MW (Kd)/pI e

Cov. rate f

Uniprot ID g

Sim. h

Average ratio i

Cel. Loc. j

TTP

UNP

BNP

Loc

RC

Pollen wall development

137

Cotton_A_15299

Chalcone synthase

129

42.81/5.37

31.77%

P30078

44.73

0.63

0.43

NDS

S

4

140

Cotton_D_gene_10017980

Pyruvate dehydrogenase E1 component subunit beta

196

39.51/5.89

39.50%

P52904

83.38

0.77

0.47

0.42

M

3

141

Cotton_D_gene_10001804

Pyruvate dehydrogenase E1 component subunit beta

194

36.19/4.75

28.74%

P52904

90.42

1.00

0.48

0.50

_

2

145

Cotton_A_16390

Enoyl-[acyl-carrier-protein] reductase

145

41.86/8.18

47.06%

P80030

83.6

0.43

0.07

-

C

2

Protein metabolic

37

Cotton_A_32277

3-isopropylmalate dehydratase small subunit

139

27.59/6.95

27.03%

Q8YX03

44.76

0.46

0.37

0.06

C

1

155

Cotton_D_gene_10025384

3-isopropylmalate dehydratase small subunit

282

27.57/6.87

25.10%

Q8YX03

44.76

1.62

0.30

1.19

C

1

39

Cotton_A_02938

Proteasome subunit alpha type-2-B

208

33.39/8.57

39.07%

Q8L4A7

93.64

0.93

0.96

1.58

_

3

168

Cotton_A_23018

Proteasome subunit beta type-1

187

24.94/7.30

68.61%

O82531

92.83

1.64

1.79

1.07

_

3

63

Cotton_A_15704

26S protease regulatory subunit 6B

296

46.86/5.27

44.47%

Q9SEI4

95.88

2.03

0.86

1.46

_

3

Pollen tube growth

138

Cotton_A_04015

Probable pectinesterase/pectinesterase inhibitor 58

175

35.79/5.06

24.30%

Q9FJ21

54.22

-

-

0.47

_

2

143

Cotton_A_28846

Probable pectinesterase/pectinesterase inhibitor 58

192

76.15/6.76

23.18%

Q9FJ21

54.22

-

-

0.50

_

2

103

Cotton_D_gene_10005074

Probable pectinesterase/pectinesterase inhibitor 58

225

19.43/4.62

43.43%

Q9FJ21

53.9

0.52

0.20

0.21

S

1

166

Cotton_D_gene_10000106

Pectinesterase PPME1

473

41.07/6.53

49.33%

Q84WM7

55.21

-

-

NDS

S

4

173

Cotton_D_gene_10000106

Pectinesterase PPME1

428

41.08/5.82

48.53%

Q84WM7

55.21

1.18

1.04

0.21

S

4

102

Cotton_A_28902

Anther-specific protein LAT52

135

19.74/4.66

43.43%

P13447

45.51

-

-

NDS

S

1

122

Cotton_A_35804

Anther-specific protein LAT52

169

20.25/4.75

27.78%

P13447

48.8

-

-

NDS

S

1

Energy and metabolism process

38

Cotton_A_33732

Triosephosphate isomerase

136

27.73/5.17

46.09%

Q9SKP6

89.84

1.20

0.96

0.03

_

2

52

Cotton_A_22450

Malate dehydrogenase

119

35.90/6.51

32.23%

Q08062

92.15

1.24

1.02

1.59

_

5

164

Cotton_D_gene_10010185

Galactose oxidase

255

70.33/6.15

35.60%

P0CS93

23.61

-

-

0.21

S

3

165

Cotton_A_14520

Galactose oxidase

242

212.1/6.07

23.04%

I1S2N3

24.26

-

-

0.10

S

2

18

Cotton_A_28004

ATP synthase subunit

217

19.56/4.66

50.00%

Q9FT52

81.55

1.09

0.72

0.65

_

3

48

Cotton_A_03219

NADH dehydrogenase [ubiquinone] flavoprotein 2

299

28.78/7.76

31.89%

O22769

86.27

0.92

0.66

1.05

M

1

99

Cotton_D_gene_10017814

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5

222

19.30/4.57

40.36%

P80266

86.49

0.95

0.61

0.59

_

5

9

Cotton_D_gene_10034077

Deoxyuridine 5′-triphosphate nucleotidohydrolase

411

18.42/6.11

72.16%

Q9STG6

79.88

1.64

0.95

2.24

_

4

10

Cotton_D_gene_10034077

Deoxyuridine 5′-triphosphate nucleotidohydrolase

86.6

18.42/6.11

38.64%

Q9STG6

79.88

1.62

0.92

1.88

_

4

32

Cotton_D_gene_10028012

Succinyl-CoA ligase [ADP-forming] subunit beta

137

45.63/6.78

55.11%

Q84LB6

87.47

1.69

0.77

1.13

M

1

43

Cotton_A_19267

Probable 6-phosphogluconolactonase 4

72.6

35.31/6.96

24.16%

A2Z3C4

73.81

1.54

0.90

1.17

C

1

55

Cotton_A_33551

Caffeic acid 3-O-methyltransferase

124

40.74/5.63

26.49%

P46484

81.35

2.00

0.87

0.78

_

2

56

Cotton_D_gene_10035451

S-adenosylmethionine synthase

299

43.49/5.59

67.43%

Q8GTL5

97.4

1.26

1.35

1.71

_

2

57

Cotton_D_gene_10028579

S-adenosylmethionine synthase 1

226

43.45/5.77

56.23%

Q9AT56

96.95

1.10

1.11

2.76

_

2

58

Cotton_D_gene_10008718

S-adenosylmethionine synthase 2

251

43.51/5.77

60.56%

Q9AT55

97.96

0.80

0.92

1.57

_

2

69

Cotton_A_16872

Probable aldo-keto reductase 1

292

49.05/8.27

17.46%

C6TBN2

66.57

1.04

0.99

1.55

_

3

70

Cotton_A_16872

Probable aldo-keto reductase 1

113

49.05/8.27

17.69%

C6TBN2

66.57

0.93

0.90

1.63

_

3

74

Cotton_D_gene_10014349

Probable mannitol dehydrogenase

151

39.52/6.08

22.80%

Q9ZRF1

76.14

0.85

1.82

1.45

_

4

85

Cotton_D_gene_10036599

Probable cinnamyl alcohol dehydrogenase 9

96.6

39.57/5.67

22.84%

P42734

79.89

2.24

2.11

3.47

_

3

Protein folding and assembly

20

Cotton_A_13598

23.6 kDa heat shock protein

220

23.48/5.22

55.77%

Q96331

62.15

1.55

NDS

-

M

5

59

Cotton_A_16089

Protein disulfide-isomerase

284

55.70/5.06

38.99%

Q9XF61

75.32

1.52

1.17

1.03

S

1

71

Cotton_A_21989

Elongation factor Tu

459

49.46/6.62

53.20%

Q9ZT91

85.93

1.11

1.48

2.25

C

5

91

Cotton_D_gene_10037266

17.3 kDa class II heat shock protein

111

17.56/6.02

66.67%

O82013

77.61

1.65

0.96

1.13

_

1

124

Cotton_A_18176

Hsp70 nucleotide exchange factor FES1

237

40.89/4.93

34.95%

Q0V4C4

36.61

0.80

0.74

0.47

_

3

Stress defense

126

Cotton_D_gene_10025297

L-ascorbate peroxidase 1

295

44.29/6.09

41.79%

Q05431

74.49

0.97

0.93

0.61

_

2

100

Cotton_A_23038

Aldehyde dehydrogenase family 2 member B4

129

58.17/7.25

28.63%

Q9SU63

77.82

1.17

0.65

0.60

M

4

170

Cotton_A_23038

Aldehyde dehydrogenase family 2 member B4

191

58.17/7.25

29.57%

Q9SU63

77.82

1.11

1.45

2.08

M

4

171

Cotton_A_23038

Aldehyde dehydrogenase family 2 member B4

559

58.17/7.25

48.02%

Q9SU63

77.82

0.53

1.46

1.52

M

4

Other functions

2

Cotton_D_gene_10029341

Glycine-rich RNA-binding protein

373

17.13/8.4

81.07%

Q03878

84.15

0.91

1.61

1.36

_

4

3

Cotton_D_gene_10029341

Glycine-rich RNA-binding protein

335

17.13/8.4

77.51%

Q03878

84.15

1.41

1.11

1.62

_

4

23

Cotton_A_35766

Pathogenesis-related protein 5

152

24.02/4.28

16.07%

P28493

63.64

-

1.51

1.02

C

4

31

Cotton_D_gene_10012588

Nuclear migration protein nudC

210

33.16/4.87

38.75%

O35685

61.18

0.94

1.15

0.57

_

5

34

Cotton_D_gene_10010187

Probable lactoylglutathione lyase

82.1

40.25/7.53

40.56%

Q8W593

78.61

1.92

1.08

0.86

C

3

35

Cotton_A_02073

Soluble inorganic pyrophosphatase 1

70.6

32.32/6.38

32.87%

Q9LXC9

80.07

1.53

0.88

0.90

C

3

44

Cotton_A_28411

Probable rhamnose biosynthetic enzyme 1

202

33.98/5.79

35.67%

Q9SYM5

82.31

1.03

0.86

2.55

_

3

51

Cotton_A_33743

Polygalacturonase QRT3

281

52.62/5.75

31.38%

O49432

63.52

0.97

1.12

2.44

C

3

83

Cotton_A_17250

Alpha-1,4-glucan-protein synthase 1

86.7

12.58/5.55

34.86%

Q9SC19

96.3

2.92

0.92

1.34

_

5

86

Cotton_D_gene_10037393

Profilin

233

14.41/5.48

42.86%

O49894

83.46

0.91

1.13

0.35

_

2

97

Cotton_A_29462

Leucine-tRNA ligase

75.1

111.1/7.00

20.18%

Q2S415

60.18

1.07

0.68

0.66

C

2

106

Cotton_A_35377

Putative pinene synthase

63.3

43.85/7.38

32.89%

P0CV97

47.09

-

-

0.18

S

4

117

Cotton_D_gene_10024340

Phosphoglycolate phosphatase

96.9

27.67/4.57

31.73%

Q5PLX6

36.47

1.05

0.64

0.64

_

3

156

Cotton_A_00485

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3

164

28.06/6.35

27.24%

Q84UV8

41.67

-

-

0.62

S

1

157

Cotton_A_00484

Nacrein-like protein C1

104

24.05/6.17

28.77%

A0ZSF6

54.05

-

-

0.28

_

2

174

Cotton_A_12497

Alpha-1,4-glucan-protein synthase

130

41.75/6.65

43.29%

P85413

93.55

1.03

0.88

0.35

_

3

19

Cotton_D_gene_10038772

Uncharacterized protein

71.5

10.81/4.81

57.29%

  

1.06

1.14

1.97

_

5

175

Cotton_D_gene_10017750

Uncharacterized protein

330

18.36/5.88

37.74%

  

0.83

1.71

NDF

_

2

  1. aSpot No corresponding to spots in protein maps.
  2. bProtein ID of the matched protein from the Cotton Genome Project (CGP, Institute of Cotton Research of CAAS) database (http://cgp.genomics.org.cn/page/species/index.jsp). The IDs started by “Cotton_A” were sequences from Gossypium arboretum genome, and “Cotton_D_gene” represent Gossypium raimondii genome.
  3. cDescription of the protein in UniProtKB.
  4. dScore obtained from Mascot for each match, and the cutoff was 62.
  5. eTheoretical molecular mass and isoelectric point.
  6. fCoverage rate, percentage of predicated protein sequence covered by matched sequences.
  7. gUniProt ID of the homolog in UniProtKB.
  8. hSimilarity between the identified protein and its homolog in UniProtKB.
  9. iAverage ratio is a ratio of the protein spots %volume ratio between the MT and WT. Ratios marked by black and italic showed p-value < 0.05 by Student’s t-test. “-”: not detectable in both maps, NDS: not detectable in the sterile MT map, NDF: not detectable in the fertile WT map.
  10. jCellular locations predicting used TargetP 1.1 Server. Loc is the locations. C, Chloroplast, M, Mitochondrion, S, Secretory pathway, “_”, Any other location. RC, Reliability Class, from 1 to 5, where 1 indicates the strongest prediction and 5 reliability classes.