Figure 3
The accumulation of H 2 O 2 at transient expression sites after inoculation with P. infestan . A. The accumulation of H2O2 was visualized by staining leaf segments transiently expressing CA-AtRop1 and DN-AtRop1 with diaminobenzidine (DAB) over time after inoculation with P. infestans. The right side of each leaf was inoculated with P. infestans, and the left side used as the control. B. The accumulation of H2O2 was quantified using the Quantity One (Bio-Rad) tool. Each value represents the average (centimeters2 ± standard deviation) of three lesions. Levels not labeled with the same letter are significantly different at the P < 0.05 level based on the ANOVA.