Figure 5
Resistance of potato AtRop1 (DN-AtRop1) transgenic lines to P. infestans . A. Four weeks old of transgenic (DN-AtRop1) potato. B. Symptoms on detached leaves of the DN-AtRop1 transgenic line after infection with P. infestans. Photographed at 96 hpi. C. Mycelial development on the leaves of DN-AtRop1transgenic lines (line 6 and line 11) after 96 hpi. The mycelium was stained with trypan blue. D. Relative quantification (RQ) by real-time PCR of lesion sites by comparing levels of the P. infestans-specific repetitive DNA sequence (as measure for fungal biomass) relative to the potato EF-1α gene (for equilibration) at 96hpi. Bars represent averages with standard deviation of three technical replicates. CK represents the wild-type potato and EV represents the potato transformed with the empty vector p1300-221.